大孔吸附树脂对莲房原花青素吸附纯化性能的研究  

ADSORPTION AND PURIFICATION OF LOTUS SEEDPOD PROANTHOCYANIDINS BY MACROPOROUS RESIN

作　　者：段玉清[1] 张海晖[1] 周密[1] 葛方胜[1] 闫永胜[1] 谢笔钧[2] 

机构地区：[1]江苏大学食品与生物工程学院,镇江212013 [2]华中农业大学食品科学技术学院,武汉430070

出　　处：《离子交换与吸附》2009年第2期114-120,共7页Ion Exchange and Adsorption

基　　金：国家自然科学基金(30270938);江苏省教育厅自然基金(05KJB550011);江苏大学人才基金(05JDG013).

摘　　要：比较了14种大孔吸附树脂对莲房中原花青素(proanthocyanidins of lotus seedpod,LSPAs)的吸附及解吸性能,在研究静态吸附的基础上,筛选出效果较好的树脂进行动态实验研究,并对所得组分LSPAs含量及其相对分子量进行初步分析。结果表明,DM130大孔吸附树脂分离纯化LSPAs效果最佳,上样浓度为2.5mg/mL,流速为3BV/h时,饱和吸附量为4～4.5个BV;当用体积分数为50%乙醇以3BV/h的流速洗脱5BV时,LSPAs的累积回收率可达96.43%,含量从22.54%提高到95.31%;经质谱分析,(M+H)分子量范围为291.1～1155.3,聚合度≤4。14 types of macroporous resins were studied in order to compare their characteristics in absorbing and desorbing proanthocyanidins of lotus seedpod （LSPAs）. Based on static adsorption, two better macroporous resins were chosen to carry out dynamic adsorption experiments, and the content and relatively molecular weight of LSPAs were analyzed. It was showed that DM130 was the optimal macroporous resin for separating and purifying LSPAs, and the optimum separating condition was as follows： LSPAs concentration 2.5mg/mL, flow rate 3BV/h, amount of saturated adsorption toward LSPAs 4-4.5BV. When the amount of elution was 5BV, 50% ethanol used as eluant, and the flowing rate was 3BV/h, the cumulation recovery of LSPAs purified by DM130 reached 96.43%, and the purity of LSPAs was the purity was increased from 22.54% to 95.31%. It was showed that the molecular weight of the purified product of LSPAs ranged from 291.1 to 1155.3 （M＋H）, and the polymerization 〈4 by further EI-MS analysis.

关 键 词：大孔吸附树脂 莲房 原花青素 分离纯化 

分 类 号：O647.3[理学—物理化学]