极低频电磁场对实验大鼠睾丸组织红外谱影响的研究  

Study on Testicle Tissue of Rats in Extremely Low Frequency Electromagnetic Fields

作　　者：张安英[1] 袁平[1] 周玉荣[1] 邓波[1] 庞小峰[1] 

机构地区：[1]电子科技大学生命科学与技术学院,成都610054

出　　处：《生物医学工程学杂志》2009年第2期248-252,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30600127)

摘　　要：研究表明极低频电磁场可能影响动物生殖功能，本文应用傅立叶变换红外（Fourier transform infrared，FTIR）光谱法分析雄性大鼠睾丸组织受高场强极低频电磁场暴露后组织结构改变情况。将雄性Wistar大鼠分为2组，其中1组暴露于电场强度E为3500-4000V／m，磁场强度B为8-10μT的极低频电磁场400d，然后观察其组织病理切片及其红外光谱的变化情况。结果发现，雄性大鼠睾丸组织受高场强极低频电磁场辐照后，其红外谱有显著变化，说明长期暴露于高场强极低频电磁场环境可能导致雄性实验动物睾丸组织中蛋白质二级结构稳定性下降，从而导致生物效应。The testicle tissue＇s pathological changes of Wistar rats in low frequency electromagnetic fields were observed and analyzed by Fourier transform infrared （FTIR） spectroscopy. The components of secondary structure were examined by use of derivative and curve-fitting techniques. The results showed some significant differences between the exposed group and the sham-exposed group on spectra. Especially, the analysis on the components of secondary structure of amide link I bond revealed a marked decrease in the ordered secondary structure of the exposed group. These findings indicate there will be damage to the testicle tissues that have been exposed to extremely low frequency electromagnetic fields for a long time.

关 键 词：傅立叶变换红外光谱 高场强 极低频电磁场 蛋白质二级结构 睾丸组织 

分 类 号：Q64[生物学—生物物理学]