早期胚胎与恶性肿瘤细胞体外共培养相互作用的实验研究  

Experimental Study on Co-culture of Early Embryo and Cancer Cells In Vitro

作　　者：张宁刚[1,2] 鄢希[1] 蒋莎莎[1] 王国平[1] 路泽军[1] 任雅琼[1] 毛咏秋[1] 阚兵[1] 罗锋[1] 

机构地区：[1]四川大学华西医院肿瘤中心生物治疗国家重点实验室,成都610041 [2]苏州大学附属二院肿瘤科,215000

出　　处：《生物医学工程学杂志》2009年第2期334-337,341,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30370550)

摘　　要：恶性肿瘤细胞与植入前小鼠胚胎的共培养是研究两者在共同环境中生物学行为变化的重要手段。取得小鼠交配后3.5d囊胚,分别与鼠源性肿瘤细胞株B16和人源性肿瘤细胞株HepG2共培养,观察胚胎的发育状况以及肿瘤细胞增殖、凋亡变化。结果发现胚胎在体外与肿瘤细胞共存的环境中能正常发育,且与对照相比其脱带率、贴壁率和扩展率均有显著提高(P<0.05),但肿瘤细胞在增殖、凋亡等生物学行为方面无明显变化(P>0.05)。实验表明早期胚胎和肿瘤细胞可以在共同的微环境中正常生长,胚胎和肿瘤细胞对生存条件的选择具有相似性;肿瘤细胞提供的生长环境可以促进胚胎的发育,这可能与肿瘤细胞提供的适宜胚胎发育的环境和两者在基因表达、生长因子及信号转导机制等生物学方面的相似性有关。The co-culture system of early embryos and cancer cells is an important means to observe the biological behavior changes of embryos and cancer cells in vitro. In this study, we co-cultured the 3. 5 dpc mouse embryo with malignant tumor cells, investigated the development of blastocyst by observing the hatchment,attachment and out- growth, observed the biological behavior changes of cancer cells in the embryonic circumstances, and detected the proliferation and apoptosis of cancer cells. Compared with the control, the embryos developed normally in the tumor environments, and the rate of hatchment, attachment and outgrowth increased significantly（P〈0.05）. However, there was no significant change of cancer ceils in morphology, proliferation and apoptosis in the co-culture system（P 〉0.05）. Under the co-culture system, the early embryo developed normally, and the cancer cells also grew well. There may be similarities between the embryo＇s and cancer cell＇s choice for living. Moreover, the growth of embryos could be promoted by cancer cells in the co-culture system. This might be related to the similarities of gene expression, growth factors and signal transduction mechanisms between embryos and cancer cells.

关 键 词：肿瘤细胞 小鼠胚胎 共培养 

分 类 号：R73-3[医药卫生—肿瘤]