重组人源β-防御素-2基因真核表达质粒转染293细胞的表达产物抗菌活性的研究  

Antimicrobial Activities of the Culture Supernatant of 293 Cells Transfected with Recombinant HBD-2 Gene Eukyriotic Expressive Plasmid

作　　者：王慧[1] 董碧蓉[1] 周焱[1] 

机构地区：[1]四川大学华西医院老年科,成都610041

出　　处：《生物医学工程学杂志》2009年第2期371-373,共3页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30472176)

摘　　要：利用分子生物学技术构建人源β-防御素-2(HBD-2)真核表达重组质粒pcDNA3.1-zeo(+)-HBD-2,经脂质体介导转染293细胞,RT-PCR检测到HBD-2基因成功转染293细胞。收集培养上清,采用肉汤对倍稀释法检测抗菌活性,结果显示培养上清对金黄色葡萄球菌有抗菌活性,而在相同条件下未检测到抗铜绿假单胞菌活性,提示人源β-防御素-2(HBD-2)可能具有优势抗金黄色葡萄球菌活性。In this study, HBD-2 eukaryotic expression recombinant plasmid peDNA 3.1-zeo（＋）-HBD-2 was constructed by the molecular cloning technologies, and 293 cells were transfeeted by liposome. The cell culture supernatant was collected and its antibacterial activities against Staphylococcus aureus ATCC25923 and Pseudomanas aeruginosa ATCC27853 were tested. The antibacterial activity against S. aureus of the culture supernatant of the HBD-2 gene transfeeted-293 cells was detected, whereas in the same condition, the antibacterial activity against P. aeruginosa was not detected. This result suggested that HBD-2 may preferentially kill Staphylococcus aureus.

关 键 词：Β-防御素-2 抗金色葡萄球菌活性 293细胞 

分 类 号：Q78[生物学—分子生物学]