特发性血小板减少性紫癜与HPA等位基因相关性研究  

Study of the relationship between idiopathic thrombocytopenic purpura and HPA

作　　者：梁晓浏[1] 王保龙[1] 上官志敏[1] 叶书来[1] 陈家萍[1] 廖艳秋[1] 朱梅红[1] 姚萍[1] 

机构地区：[1]安徽医科大学附属省立医院输血科,安徽合肥230001

出　　处：《中国输血杂志》2009年第3期207-210,共4页Chinese Journal of Blood Transfusion

摘　　要：目的探讨HPA-1—6、15等位基因与特发性血小板减少性紫癜(ITP)的相关性。方法采用聚合酶链反应序列特异性引物(PCR-SSP)法,对ITP组(n=25)和健康对照组(n=25)进行HPA-1—6、15共7个抗原系统基因分型,采用ELISA法和微柱凝胶法检测2组人群血小板吸附抗体及血小板游离抗体。结果ITP组HPA-3的基因频率明显异于对照组(P<0.01),其中HPA-3 a基因频率高于对照组,HPA-3b基因频率低于对照组,其余等位基因在2组间未发现有明显区别(P>0.05);ITP组血小板游离抗体检测到17例阳性(68%),吸附抗体检出21例(84%),对照组血小板抗体未检出。结论本次研究显示HPA-3抗原系统和ITP的发病可能存在一定的关联;血小板吸附抗体在ITP中检出率明显高于游离抗体。Objective To observe the relationship between HPA-1--5, 6,15 alleles and idiopathic thrombocytopenic purpura. Methods Polymerase chain reaction-specific sequence primers （PCR-SSP） was used to gcnotype HPA-1-5, 6, and 15 in the ITP group （n = 25 ） and the health control group （n = 25）. The platelet adsorbed and free antibodies in the two groups were detected by ELISA and microcolumn gel immunoassay. Results The allele frequencies of HPA-3 was significantly different between the ITP group and control group （P 〈0.01）, and the frequency of HPA-3a in ITP group was higher than that in control group, and the frequency of HPA-3b was lower than that in the control group, the frequencies of other alleles showed no significant difference between the two groups（ P 〉 0. 05 ）. The platelct free antibodies were detected in 17 ITP samples（68% ） and the adsorbed antibodies were detected in 21 ITP samples（84% ）. No platelet antibodies were detected in the control group. Conclusion There was a possible association between the HPA-3 allele and ITP. The detection rate of platelet adsorbed antibodies is significant higher than that of platelct free antibodies.

关 键 词：血小板减少性紫癜 特发性 HPA 等位基因 基因多态性 

分 类 号：R558.9[医药卫生—血液循环系统疾病] R457.11[医药卫生—内科学]