脑缺血大鼠大脑皮层存活素及血管内皮生长因子的表达  

Expression of Survivin and VEGF in the cerebral ischemia rat

作　　者：薛慎伍[1] 张建中[2] 郑虹[1] 王辉[1] 张兆岩[1] 孔顺利[1] 

机构地区：[1]济南军区总医院干部四科,山东济南250031 [2]解放军第306医院病理科,北京100101

出　　处：《中华保健医学杂志》2009年第2期102-104,F0003,共4页Chinese Journal of Health Care and Medicine

摘　　要：目的探讨存活素(Survivin)对大鼠脑缺血区神经细胞凋亡抑制的影响和介导血管内皮生长因子(VEGF)阳性表达的微血管修复再通的作用。方法18只Wistar大鼠随机分为缺血组、盐水组、假手术组、正常组,建立局灶脑缺血模型,采用PV-6000两步免疫组织化学染色法检测,光学显微镜观察大脑皮层Survivin及VEGF阳性细胞并计数。结果缺血组、盐水组、假手术组、正常组Survivin阳性表达数分别为67.32±7.34、23.63±4.63、18.21±2.17、2.56±0.11,VEGF阳性表达数分别为47.83±5.04、19.33±4.10、14.12±1.21、1.47±0.03,缺血组Survivin和VEGF阳性表达明显高于其他3组(P<0.05),缺血组大脑皮层神经元和室管膜上皮细胞等处Survivin阳性细胞表达明显。结论脑缺血大鼠大脑皮层Survivin阳性细胞的表达可抑制或减慢细胞调亡,介导VEGF促进微血管修复的形成过程。Objective To study the expression of Survivin and VEGF to neurocyte apoptosis of ischemic region and their influenceto capillary repair. Methods 18 Wistar rat were randomly derided into ischemic group,normal contral group,normal saline group,sham operated group,the partial cerebral ischemia model was induced,the Survivin and VEGF positively staining cell was counted by light microscope after using immunohistochemistry kit by two steps PV-6000 immunohistochemistry staining method. Results The positive staining cells of survivin were 67.32±7.34,2.56±0.11,23.63 ±4.63 ,18.21±2.17 , in ischemie group,normal control group,normal saline group,sham operated group,respectively,and those with positively stainting of VEGF were 47.83±5.04, 1.47 ±0.03 ,19.33 ±4.10 ,14.12 ±1 .21, the Survivin and VEGF positive staining cell in ischemie group were significantly higher than those of the other three groups, （P〈0.05）. The expression of Survivin masculine eytes was obvious in the cerebral cortex neuron and the endyma cedyma epithelialis of the ischemic group. Conclusions The expression of Survivin masculine cytes of the cerebral ischemia rat＇s cerebral cortex can inhibit apoptosis,and mediate the VEGF to promote the process of the angiopoiesis.

关 键 词：脑缺血 大鼠 存活素 血管内皮生长因子 

分 类 号：R743.3[医药卫生—神经病学与精神病学]