犬骨髓基质细胞体外培养和诱导分化的实验研究  

In Vitro Cultivation and Induced Differentiation of Dogs' Bone Marrow Stromal Cells

作　　者：万蕾蕾[1] 裴庆国[1] 蔡晓清[1] 宋萌[1] 

机构地区：[1]上海交通大学附属第一人民医院口腔科,上海200080

出　　处：《口腔医学研究》2009年第2期149-152,共4页Journal of Oral Science Research

基　　金：上海市卫生局基金资助项目(编号:2006051)

摘　　要：目的:观察犬骨髓基质细胞在体外培养的条件及生物学特性,对其进行鉴定。方法:取犬骨髓分离培养骨髓基质细胞(BMSC)传代后,倒置相差显微镜观察其形态,免疫组化方法加以鉴定,生化检测Vonkossa染色鉴定其成骨能力。结果:原代培养20d后可分离得到骨髓基质细胞BMSC,生长形态相对稳定,具有成骨能力。结论:BM-SC具有贴壁生长特征,易分离扩增,增殖速度快,诱导分化具有成骨能力,可用于骨缺损的修复治疗。Objective：To observe in vitro culturing condition and biological chatacteristics of bone marrow stromal cells（BMSC） of dogs and identify the cells. Methods：Bone marrow of dogs were separated and the BMSC were cultured. The morphology of the cells was studied with a phase contrast microscope and the living characteristics after subculture of cells were observed. Result：BMSC could be observed after 20 days primary culture. The living biological chatacteristics were quite stable,and owned the bone formation activity. Conclusion：BMSC can be easily separated and proliferated fastly,which might be used to repair bone defect.

关 键 词：骨髓基质细胞 体外培养 诱导分化 

分 类 号：R780.2[医药卫生—口腔医学]