3′-大豆苷元磺酸钠对心肌缺血/再灌注损伤及抗氧化作用的影响  

The therapeutic effects of 3′-daidzein sulfonate sodium on isolated myocardial ischemia/reperfusion injury and its relation with anti-oxidation

作　　者：黄志华[1] 李良东[1] 肖海[1] 黄贤华[2] 曾靖[2] 

机构地区：[1]赣南医学院基础医学院,江西赣州341000 [2]赣南医学院药学院,江西赣州341000

出　　处：《中国药理学通报》2009年第4期481-483,共3页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30560173);江西省自然科学基金资助项目(No0640003)

摘　　要：目的利用离体心脏灌流模型,研究3′-大豆苷元磺酸钠对离体心脏缺血/再灌注损伤的保护作用,并探讨其抗氧化作用的机制。方法应用Langendorff离体心肌缺血/再灌注损伤模型,在灌流液中加入高、中、低剂量的3′-大豆苷元磺酸钠,测定再灌注过程中冠脉流量、左心室收缩压的变化;比色法测定心肌组织中LDH、SOD、GSH-Px的活性。结果3′-大豆苷元磺酸钠可使缺血/再灌注后心脏灌流量及左心室收缩压增大,使心肌组织中LDH、SOD、GSH-Px的活性升高。结论3′-大豆苷元磺酸钠对离体心脏缺血/再灌注损伤具有保护作用,其保护机制与其增强抗氧化作用有关。Aim To explore the therapeutic effects of 3＇-daidzein sulfonate sodium （DSS） on isolated myocardial ischemia/reperfusion injury and the relation of its mechanism to anti-oxidation. Methods An isolated rat myocardial ischemia/reperfusion injury was made, and the injury was treated with DSS. Following the treatment, the coronary blood flow levels, left ventricular systolic pressure, and activities of enzymes （ LDH, SOD, GSH-Px） were measured. Results After treat-merit, the isolated myocardial tissue showed an increase in the volume of perfusion and an increase in the enzymatic activities of LDH, SOD, GSH-Px in the myocardial tissue. Conclusion DSS has a therapeutic effect on isolated myocardial ischemia/reperfusion injury because it enhances anti-oxidative activity in myocardial tissue.

关 键 词：3-大豆苷元磺酸钠 缺血/再灌注损伤 抗氧化作用 

分 类 号：R-332[医药卫生] R284.1