埃他卡林对异丙肾上腺素诱导心肌细胞肥大的影响  

Effect of iptakalim on prevention isoprenaline induced cardiomyocytes hypertrophy

作　　者：高杉[1] 龙超良[2] 王汝欢[2] 汪海[2] 

机构地区：[1]安徽医科大学药理学教研室,安徽合肥230032 [2]军事医学科学院毒物药物研究所,北京100850

出　　处：《中国药理学通报》2009年第4期484-487,共4页Chinese Pharmacological Bulletin

基　　金：国家高技术研究发展计划(863计划)重大专项资助(No2002AA2Z3137)

摘　　要：目的在原代培养的新生大鼠心肌细胞上观察埃他卡林(iptakalim,IPT)对异丙肾上腺素诱导心肌细胞肥大的影响。方法采用SD大鼠乳鼠原代心肌细胞培养法,Lowry法测定心肌细胞总蛋白含量;激光共聚焦显微镜观察心肌细胞内游离Ca2+浓度。结果①剂量为1×10-5mol.L-1的异丙肾上腺素(isoprenaline,ISO)能有效地诱导心肌细胞肥大;②IPT能剂量依赖性抑制ISO诱导心肌细胞总蛋白含量和心肌细胞游离Ca2+浓度增加。结论IPT能明显抑制ISO诱导的心肌细胞总蛋白含量的增加,其机制可能与抑制Ca2+内流有关。Aim To investigate the effect of iptakalim on neonatal cardiomyocytes hypertrophy induced by isoprenaline （ISO）. Methods Hypertrophy in neonatal rat cardiac myocytes was established via culture with ISO. Total protein content was measured by Lowry＇s method. The fluorescence intensity of intracellular Ca^2＋ was detected respectively with Fluo-3/AM loading by the laser confocal microscopy. Results ① 1 × 10^ -5 mol · L^-1 ISO can activated β-AR completely; ② the total protein of cardiomyocytes and intracellular [ Ca2^ ＋ ]i was markedly decreased by treatment with IPT and represented a dose-dependent manner. Conclusion IPT could inhibit the increase of protein content of cardiomyocytes induced by ISO, which may be contributed to the decease of intracellular Ca^2＋ concentration.

关 键 词：心肌细胞肥大 埃他卡林 异丙肾上腺素 激光共聚 焦显微镜 

分 类 号：R-332[医药卫生] R322.2