MRI监测兔VX2瘤脑脊液源性转移与VEGF表达的相关性研究  

Dynamic Contrast Enchanced MRI Study of Cerebrospinal Fluid Metastasis and its Relationship with VEGF Expression

作　　者：李康安[1] 张贵祥[1] 张峰[1] 赵京龙[1] 李玉洁[1] 王夕富[1] 胡运胜[1] 

机构地区：[1]上海交通大学附属第一人民医院放射科,上海市武进路85号上海200080

出　　处：《中国医学计算机成像杂志》2009年第2期180-185,共6页Chinese Computed Medical Imaging

基　　金：国家自然科学基金资助(30571781);上海市科委重点基金资助(07jc14032)

摘　　要：目的:分析兔VX2瘤脑脊液源性转移动物模型中血管内皮生长因子(VEGF)的表达及其变化规律;MRI动态监测动物模型的形态学变化,分析其参数与肿瘤VEGF表达水平的相关性。方法:30只新西兰大白兔分两组。每次MRI检查前,分别抽取实验兔的脑脊液和血液样品做VEGF的酶联免疫吸附法(ELISA)测定。MRI扫描后处死所扫描实验兔,获取病理标本,行HE染色及VEGF免疫组化染色。结果:经t检验,参数SLE及MER在VEGF表达分组中有显著统计学差异。实验组免疫组化在肿瘤细胞的细胞质和细胞膜以及部分细胞核中均可见VEGF阳性颗粒,对照组呈阴性。DCE-MRI参数与VEGF表达经Spearman's等级相关法分析,SLE与肿瘤组织免疫组化VEGF表达呈正相关(P<0.01),而MER与肿瘤组织免疫组化VEGF表达呈负相关(P<0.05)。结论:在兔VX2瘤脑脊液源性转移中,VEGF呈高表达,动态增强磁共振成像可有效地监测肿瘤的生物学行为,为评价脑脊液源性转移是否采用抗血管治疗及判定治疗效果提供可靠的影像学检测手段。Purpose： To evaluate the VEGF expression level in the animal models . To analyze the correlation of DCE - MRI with VEGF expression. Methods： Thirty New Zealand rabbits were divided into 2 groups. DCE - MRI was performed on different days. MER and SLE were calculated. Before DCE - MRI examination of the rabbit, the VEGF level of plasma and CSF were evaluated by ELISA method. After DCE - MRI examination, the rabbits were sacrificed randomly and the corresponding tumor specimens were harvested. HE staining, VEGF immunohistochemical staining were carried out. Results： VEGF positive staining was localized in tumor cytoplasms and cell membranes, and some epithelial cells also had a positive expression. VEGF immunohistochemical score bore a positive correlation with SLE（P 〈 0.01） by rank correlation statistical method. The VEGF expression in the plasma and CSF showed a positive correlation with SLE （P 〈 0.05） by rank correlation statistical method. Conclusions： The VEGF expression level in the tumor , plasma and CSF in the cerebrospinal fluid metastasis were higher than that in the normal tissue. DCE - MRI had a good correlation with VEGF expression of cerebrospinal fluid metastasis.

关 键 词：动态增强磁共振成像 酶联免疫吸附法 血管内皮生长因子 脑脊液 转移 

分 类 号：R73-3[医药卫生—肿瘤]