Wistar大鼠腹主动脉瘤模型的建立及改进  

A rat models of abdominal aortic aneurysms

作　　者：许文平 邱健[2] 詹纯列[2] 何柏身 马国亮 谢志泉[2] 

机构地区：[1]驻香港部队医院,广东深圳518048 [2]广州军区广州总医院心内科,广东广州510010

出　　处：《医学研究生学报》2009年第4期347-349,I0001,共4页Journal of Medical Postgraduates

基　　金：广东省医学科学基金资助项目(批准号:A2005505)

摘　　要：目的:腹主动脉瘤(AAA)是一类死亡率极高的主动脉疾病。为研究AAA的发病机制及治疗措施,必须建立合适的病理模型。文中研究建立简便、迅速、稳定的Wistar大鼠AAA模型。方法:10周龄雄性Wistar大鼠30只,随机分成实验组及对照组(各15只),实验组采用缩短弹力酶灌注时间的改进建模方法,对照组采用传统建模方法。14 d后计算2组动物的存活率、腹主动脉瘤的形成率,制作腹主动脉石蜡及电镜切片,观察其形态学变化。结果:对照组大鼠手术存活率为60%,存活大鼠AAA形成率为89%;实验组大鼠存活率为87%,存活大鼠AAA形成率为85%;两组大鼠均可见腹主动脉明显扩张,动脉壁变硬,部分区域可见内膜撕裂,中膜弹力纤维减少或消失,外膜炎性细胞浸润明显。结论:改进后的30 min弹力酶灌注法可用于AAA大鼠模型的制作,动物成活率及AAA形成率均较高。是一种简便、迅速、稳定的腹主动脉瘤Wistar大鼠建模方法。Objective： Abdominal aortic aneurysm（AAA） is a kind of abdominal aortic disorders with very high mortality.This paper reports the innovated methods for a simple,quick,stable and high-successed-rate model of abdominal aortic aneurysms（AAA）.Methods： Wistar rats were divided into control group and improving method group,and AAA models were made with traditional and modified method respectively.The two methods were compared with formation and mortality.Results： Both of the methods may induce AAA.The mortality of control group rats was 40 percents,modified method group was 14 percent.Light microscopy showed that the development of AAA in vehicle-treated rat was accompanied by marked thickening of the aortic wall,a dense transmural inflammatory response,and complete destruction of the elastic media.Conclusion： Modified method is a simple,quick,stable and high-success-rate model of abdominal aortic aneurysms.

关 键 词：腹主动脉瘤 动物模型 

分 类 号：R732.21[医药卫生—肿瘤]