不同免疫背景小鼠G422胶质瘤颅内移植瘤模型的建立  

Establishment of Introcranial G422 Glioma Models in Mice with Different Immune Backgrounds

作　　者：程迎新[1] 杜鹏[1] 李飞[1] 许桂莲[2] 李梅[1] 肖虹[1] 林江凯[1] 朱刚[1] 王宪荣[1] 冯华[1] 

机构地区：[1]第三军医大学西南医院神经外科/中国人民解放军神经系统疾病微创诊治专科中心,重庆400038 [2]第三军医大学免疫学教研室,中国人民解放军免疫学研究所,重庆400038

出　　处：《中国神经肿瘤杂志》2009年第1期45-49,共5页Chinese Journal of Neuro-Oncology

摘　　要：背景与目的:胶质瘤治疗效果差,是神经外科领域研究的难点。本研究建立正常免疫(Balb/c小鼠)、T细胞免疫缺陷(裸小鼠)和补体功能缺陷(补体C3基因敲除小鼠)三种不同免疫背景的小鼠G422胶质瘤颅内移植瘤模型,并观察肿瘤的生长特点。方法:将小鼠源性G422胶质瘤细胞种植入Balb/c小鼠、裸小鼠和补体C3基因敲除小鼠脑内,观察肿瘤种植后三种小鼠的生存期、成瘤率、肿瘤生长情况及病理学特性。结果:Balb/c鼠、裸小鼠、补体C3基因敲除小鼠三种小鼠脑内种植G422胶质瘤细胞后,成瘤率分别为90%、100%、100%。Balb/c荷瘤鼠平均生存期最长[(44.3±6.0)d],裸小鼠次之[(24.8±4.8)d],补体C3基因敲除小鼠最短[(18.6±5.2)d],肿瘤体积增大至34.29mm3需要的时间分别为35d、21d、14d;组织病理学观察显示G422胶质瘤细胞脑内种植后可保持胶质瘤的肿瘤特征。结论:采用G422小鼠胶质瘤细胞种植入Balb/c小鼠、裸小鼠、补体C3基因敲除小鼠脑内,可建立具有不同免疫背景的胶质瘤动物模型。BACKGROUND ＆ OBJECTIVES： Establishment of ideal animal model is critical in the research of gliomas. In this article, we explored the growth of brain gliomas in mice with different immune backgrounds. METHODS： Murine G422 glioma cells were stereotaxically implanted into the left parieto-oceipital lobes of Balb/c mice, nude mice and complement C3 knockout mice. The survival of the mice and the size of tumor were all observed. Tumor was confirmed with HE staining and immunohistochemistry for glial fibtillary acidic protein （GFAP）. RESULTS： Xenografts were successfully established in 90% of Bal b/c mice, 100% of nude mice, and 100% of complement C3 knockout mice respectively. The mean survival of the tumor-beating mice were （44.3 ± 6.0） d for Bal b/c, and （24.8 ± 4.8） d for nude mice and （18.6 ± 5.2） d for complement C3 knockout mice respectively. The average time for tumors to reach the volume of 34.29 mm^3 was 35 d for Balb/c, 24 day for nude mice and 14 d for complement C3 knockout mice respectively. CONCLUSIONS：We have suceessftdly established three different G422mouse model with immunel background.

关 键 词：脑胶质瘤 补体 免疫 动物模型 

分 类 号：R739.41[医药卫生—肿瘤]