《金匮要略》不同方药对肺纤维化模型早期阶段(7天)肺、脑组织NE、DA、5-HT含量的影响  

Effects of different TCM prescriptions of JinKuiYaoLve on the levels of Noradrenaline(NE),Dopamine(DA) and 5-hydroxytryptamine(5-HT) in lung and brain tissues of pulmonary fibrosis in the early stages

作　　者：宋建平[1] 李伟[1] 李瑞琴[1] 张瑞[1] 秦俊莲[1] 徐建明 田黎[3] 马莉娜[4] 张丽敏[1] 

机构地区：[1]河南中医学院,郑州450008 [2]河南煤炭总医院,郑州450002 [3]广州中医药大学,广州510405 [4]河南职工医学院,郑州451191

出　　处：《中华中医药杂志》2009年第5期568-571,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：河南省自然科学基金项目资助(No.0411044100)

摘　　要：目的:观察《金匮要略》不同方药对肺纤维化模型早期阶段(7天)肺、脑组织去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)含量的影响,探讨中药作用机制即肺纤维化早期阶段中医方证本质。方法:Wistar大鼠随机分组,除正常组外,其余各组均采用平阳霉素复制肺纤维化模型,用药组造模后第2天开始用药,7d后处死动物,比较各组肺、脑组织NE、DA、5-HT含量。结果:模型组肺组织NE含量高于正常组、瓜蒌薤白汤组和泼尼松组,5-HT含量低于肾气丸组和泼尼松组,肾气丸组DA含量低于瓜蒌薤白汤组;模型组脑组织NE含量高于正常组,DA含量低于正常组、瓜蒌薤白汤组、麦门冬汤组,大黄虫丸组5-HT含量高于其他各组(P<0.05)。结论:肺纤维化早期阶段肺、脑组织NE含量增高,脑组织DA含量降低;瓜蒌薤白汤能阻止肺组织NE含量的增高与脑组织DA含量的降低,麦门冬汤能阻止脑组织DA含量的降低,大黄虫丸能提高脑组织5-HT含量,肾气丸能降低肺组织5-HT含量。上述方药可能通过影响肺、脑组织NE、DA、5-HT含量干预肺纤维化早期阶段病变,肺、脑组织NE、DA、5-HT含量变化可能与中医方证本质有关。Objective： To observe the effect of different TCM prescriptions of JinKuiYaoLve on levels of noradrenaline（NE）, dopamine（DA） and 5-hydroxytryptamine（5-HT） in lung and brain tissues of pulmonary fibrosis in the early stages. To investigate the effect and mechanism of TCM prescriptions and the nature of prescription-syndrome. Methods： The wistar rats were divided into groups randomly. Pulmonary fibrosis was induced by bleomycin A5 in all groups except normal group. Rats in drug group were killed after 7 days＇ treatment. The levels of NE, DA and 5-HT in the lung and brain tissues were detected. Results： The level of NE in the lung tissue of model group was higher than that of normal group, Gualouxiebai decoction（GLXB） group and prednisone group. The level of 5-HT of model group was lower than that of Shenqi PilIs（SQW） group and prednisone group. The level of DA of SQW group was lower than that of GLXB group. The level of NE in the brain tissue of model group was higher than that of normal group, but the level of DA was lower than that of normal group, GLXB group and Maimendong decoction（MMD） group. The level of 5-HT of Dahuangzhechong pills （DHZCW） group was higher than that of other groups（P〈0.05）. Conclusion： The level of NE in the lung and brain tisues of rats with pulmonary fibrosis increased and that of DA in the brain tissue decreased in the early stage. GLXB can hold this change. MMD can prevent the decrease of DA level in brain tissue. DHZCW can improve the level of 5-HT. SQW can lower 5-HT level in lung tissue. These prescriptions may influence the pathological damage of pulmonary fibrosis in the early stage by regulating the level of NE,DA and 5-HT in lung and brain tissues, which may be probably associated with the nature of prescription-syndrome of TCM.

关 键 词：肺纤维化 瓜蒌薤白汤 大黄廑虫丸 肾气丸 麦门冬汤 去甲肾上腺素 多巴胺 5-羟色胺 

分 类 号：R222[医药卫生—中医基础理论] R259[医药卫生—中医学]