LY294002抑制PI3K/Akt信号通路对人胃癌SGC-7901细胞的影响  

Effects of inhibition of PI3K/Akt signal pathway by LY294002 on human gastric carcinoma SGC-7901 cells

作　　者：郭花[1] 朱金水[1] 张强[1] 王红建[1] 王龙[1] 

机构地区：[1]上海交通大学第六人民医院消化内科,上海200233

出　　处：《上海交通大学学报（医学版）》2009年第4期374-377,共4页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的探讨低氧环境下PI3K抑制剂2-（4-吗啉基）-8-苯基-4氢-1-苯并吡喃-4-酮（LY294002）对人胃癌SGC-7901细胞中磷脂酰肌醇3-激酶（PI3K）／丝氨酸苏氨酸激酶（Akt）信号通路、细胞生长、细胞凋亡及低氧诱导因子-1α（HIF-1α）mRNA表达的影响。方法应用LY294002作用于低氧环境中的人胃癌SGC-7901细胞（低氧＋LY294002组），采用CCK-8试剂盒检测SGC-7901细胞生长；流式细胞仪检测细胞凋亡百分比；Western blotting检测P13K和p-Akt蛋白表达；RT—PCR检测HIF-1α mRNA表达。以低氧环境中未加LY294002的细胞作为对照（低氧组）。结果LY294002能抑制低氧环境中SGC-7901细胞增殖，抑制效果在药物作用后第2～6天时最为明显（P〈0．05），低氧＋LY294002组中细胞凋亡百分比明显高于低氧组（P〈0．01）。LY294002能明显抑制PI3K、p-Akt蛋白以及HIF-1α mRNA的表达，与低氧组比较差异均有统计学意义（P〈0．05）。结论LY294002能抑制低氧环境中人胃癌SGC-7901细胞生长，促进细胞凋亡，抑制PI3K／Akt信号通路及其下游HIF-1α mRNA的表达。Objective To investigate the effects of PI3K inhibitor （LY294002） on PI3K/Akt signal pathway, cell growth, cell apoptosis and expression of hypoxia inducible factor （HIF）-1α mRNA in human gastric carcinoma SGC-7901 cells in hypoxia enviroment. Methods Human gastric carcinoma SGC-7901 cells in hypoxia enviroment were treated with LY294002 （hypoxia ＋ LY294002 group）. CCK-8 kit was applied to evaluate the growth of SGC-7901 cells, flow cytometry was adopted to determine the cell apoptosis rate, Western blotting was employed to detect the expression of PI3K and p-Akt protein, and RT-PCR was used to detect the expression of HIF-1α mRNA. SGC-7901 cells in hypoxia enviroment without LY294002 were served as control （hypoxia group）. Results LY294002 inhibited the growth of SGC-7901 cells in hypoxia environment, and the inhibition effect was most significant from d2 to d6 after treatment （P 〈0.05）. Cell apoptosis rate in hypoxia ＋ LY294002 group was significantly higher than that in hypoxia group （P 〈 0.01 ）. LY294002 inhibited the expression of PI3K, p-Akt protein and HIF-1α mRNA, and the difference was significant when compared with hypoxia group （ P 〈 0.05）. Conclusion LY294002 could inhibit the growth of SGC-7901 cells, increase cell apoptosis, and inhibit PI3K/Akt signal pathway and HIF-1α mRNA expression in hypoxia environment.

关 键 词：LY294002 磷脂酰肌醇3-激酶 丝氨酸苏氨酸激酶 低氧诱导因子-1Α SGC-7901细胞 

分 类 号：R735.2[医药卫生—肿瘤]