机械损伤对拟南芥莲座叶芥子油苷含量和组成的影响  

Effect of mechanical wounding on glucosinolate content and composition in rosette leaves of Arabidopsis thaliana

作　　者：田云霞[1] 戴绍军[1] 陈思学 阎秀峰[1] 

机构地区：[1]东北林业大学林木遗传育种与生物技术教育部重点实验室,东北林业大学生命科学学院,哈尔滨150040 [2]Department of Botany, University of Florida, Gainesville, FL 32611, USA

出　　处：《生态学报》2009年第4期1647-1654,共8页Acta Ecologica Sinica

基　　金：国家自然科学基金海外青年学者合作研究基金资助项目(30528013);国家自然科学基金资助项目(30670325);新世纪优秀人才支持计划资助项目(NCET-05-0328)

摘　　要：植物可以利用体内次生代谢产物的变化来抵御昆虫取食和机械损伤。芥子油苷是拟南芥的主要次生代谢产物。通过剪刀剪取叶片(40%面积)对温室培养的拟南芥幼苗莲座叶进行机械损伤处理,观察机械损伤后8个时间点拟南芥叶片中不同种类芥子油苷含量和组合模式的变化。结果表明机械损伤后3h叶片中芥子油苷总含量开始明显上升,脂肪族和吲哚族芥子油苷含量在损伤后3h也都显著高于损伤前。在检测到的12种芥子油苷中,4-甲基亚磺酰丁基芥子油苷(4-methylsulphinylbutylGS,4MSOB)的含量最多,占芥子油苷总量的48.5%,并且在损伤3h后含量增加。4MSOB含量的变化成为影响莲座叶中芥子油苷组合模式的主导因素。其它各种芥子油苷在损伤后不同时间点的变化也存在差异。The concentration and composition of plant secondary compounds are well known to confer plant resistance to herbivores,pathogens and mechanical wounding.Glucosinolates（GSs）are a major group of nitrogen-and sulfur-containing secondary metabolites of Arabidopsis thaliana.The effect of mechanical wounding on GS profile in A.thaliana rosette leaves was reported in this study.A.thaliana ecotype Columbia（Col-0）was grown in a growth chamber.At the twenty-leaf stage,the rosette leaves were wounded by cutting approximately 40% leaf area across the apical lamina with scissors.The leaves were harvested at 0.25,0.5,1,1.5,3,6,9 and 24 h after the treatment.GSs were extracted from rosette leaves.The dominant GSs were analyzed by HPLC.Wounding by scissors tend to increase GS contents.At 3,6 and 9 h after treatment,total GS content increased significantly.The contents of both aliphatic GSs and indole GSs were induced at 3h after wounding,e.g.,the aliphatic GS levels were increased up to 3.53 fold.The content of 4-methylsulphinylbutyl GS（4MSOB）in rosettes was the highest among all the 12 GSs detected,accounting for 48.5% of total content of GSs.The increase in the content of 4MSOB after wounding is probably the main factor affecting the GS profile in rosette leaves of A.thaliana.Mechanical wounding also changed the proportion of GSs in rosette leaves.The proportion of aliphatic GSs was significantly increased from ca.66% to 71%-75% at 1,3,6,and 9 h after treatment.The proportions of 4MSOB and other GSs displayed significant changes after wounding.Collectively,our results indicate that mechanical wounding regulates glucosinolate profiles in A.thaliana leaves.Further studies of the molecular mechanisms underlying the regulation will lead to advanced understanding of plant defence and interaction with the environment.

关 键 词：芥子油苷 机械损伤 拟南芥 

分 类 号：Q946[生物学—植物学]