甲基苯丙胺慢性中毒对脑纹状体区损伤作用的初步研究  

Damage of striatum during chronic toxicity of methamphetamine: a preliminary research

作　　者：刘增甲[1] 宋健文[1] 王慧君[1] 乔东访[1] 谭晓辉[1] 

机构地区：[1]南方医科大学法医学系,广州510515

出　　处：《中国新药杂志》2009年第8期733-736,共4页Chinese Journal of New Drugs

基　　金：国家自然科学基金(30572090)

摘　　要：目的：建立大鼠甲基苯丙胺（methaphetamine,MA）慢性中毒模型,探讨MA长期应用对脑纹状体区的损伤作用。方法：Wistar大鼠30只,雄性,体重180-220 g,随机分为对照组（A）和实验组（B）。B组从小剂量开始腹腔注射MA,逐渐加量,共用4周;A组腹腔注射等量生理氯化钠溶液。电子体温计监测体温变化;TUNEL法观察纹状体内神经元凋亡情况;高效液相色谱检测纹状体内多巴胺（dopamine,DA）含量。结果：MA引起大鼠行为活动明显增多;纹状体区神经细胞凋亡数量显著增多及DA含量明显下降。结论：MA慢性中毒对大鼠脑组织纹状体有明显的毒性损伤,可引起大鼠行为改变,纹状体神经元大量凋亡及DA含量显著下降。Objective： To establish a rat model of chronic toxicity of methamphetamine （MA） and to explore MA-induced chronic neurotoxicity on striatum. Methods： Thirty male rats, weighting 180 -220 g, were randomly divided into 2 groups, and treated with either gradually increasing doses of MA or saline （control） for 4 weeks. The body temperature was monitored; the changes in the behaviors were observed and graded. Neuron apoptosis was detected by TUNEL straining. The contents of the dopamine （DA） were detected with HPLC. Results： The locomotor activities increased remarkably and the number of apoptotic neurons in the striatum increased obviously after MA administration. The contents of dopamine in the striatum decreased obviously. Conclusion： Chronic application of MA induces obvious damage of the striatum, dysfunction of behaviors, neuron apoptosis and decrease in the contents of striatum dopamine.

关 键 词：甲基苯丙胺 慢性中毒 凋亡 多巴胺 

分 类 号：R972.6[医药卫生—药品] R99[医药卫生—药学]