survivin启动子的克隆及其在前列腺癌细胞系中的活性鉴定  

Cloning of tumor-specific survivin promoter and the identification of its transcriptional activities in human prostatic carcinoma

作　　者：骆晓梅[1] 张南征[2] 刘家云[3] 苏明权[3] 郝晓柯[3] 

机构地区：[1]中国人民解放军第九七医院检验科,江苏徐州221004 [2]中国人民解放军第九七医院消化内科,江苏徐州221004 [3]第四军医大学西京医院全军临床检验医学中心,陕西西安710032

出　　处：《徐州医学院学报》2009年第4期215-217,共3页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金（30500433）

摘　　要：目的克隆survivin启动子片段,并检测其在人前列腺癌细胞中的转录活性,为该启动子在前列腺癌靶向基因治疗中的应用提供实验依据。方法PCR方法扩增survivin基因启动子S1pro和S2pro,克隆入pGL3-Basic,分别构建重组质粒pGL3-S1pro和pGL3-S2pro,脂质体转染前列腺癌细胞和Chang liver肝细胞,检测sur-vivin启动子在细胞中的转录活性。结果survivin基因启动子在前列腺癌细胞中均具有较强活性,其中S2pro活性明显高于S1pro,达到CMV启动子活性的三分之一。结论survivin启动子在前列腺癌细胞中具有较强启动活性,有可能成为新的前列腺癌靶向性基因治疗工具。Objective To clone DNA sequence of survivin promoter, and detect its transcriptional activities in human prostate cancer cells. Methods The fragment of survivin promoter was acquired by PCR amplification and was inserted into pGL3 - Basic to reconstruct recombinant plasmids pGL3 - S1pro and pGL3 - S2pro, respectively. The recon- structed plasmids were transiently transfected into human prostate cancer cell lines LNCap, PC -3 and normal Chang liver cells. The transcriptional activities of survivin promoter in various cell s were determined by detection of the expression of luciferase. Results Survivin promoter had transcriptional activities in all prostate cancer cell lines and the transcrip- tional activity of the S2pro was much higher than the S1pro and reached a level of one third of the transcriptional activity of the CMV promoter. Conclusion The survivin promoter cloned in this study was more highly activated in prostate cancer cells than in normal cell s, which might enable it to be a potential candidate promoter in the gene therapy of prostate cancer.

关 键 词：前列腺癌 基因 SURVIVIN 启动子 基因治疗 

分 类 号：R737.25[医药卫生—肿瘤] R730.3[医药卫生—临床医学]