白介素-4 C590T基因多态性与血清白介素-4水平及动脉粥样硬化性血栓性脑梗死的关系  

Aassociation between gene polymorphism of interleukin-4 C590T and serum level of interleukin-4 and atherothrombotic cerebral infarction

作　　者：唐震宇[1] 杨期东[2] 周琳[2] 张永利[2] 申向民[2] 

机构地区：[1]南昌大学第二附属医院神经内科,330006 [2]中南大学湘雅医院神经内科

出　　处：《临床神经病学杂志》2009年第2期87-89,共3页Journal of Clinical Neurology

摘　　要：目的探讨白介素(IL)-4C590T基因多态性与血清IL-4水平以及动脉粥样硬化性血栓性脑梗死(ATCI)的关系。方法采用PCR-限制性片段长度多态性(PCR-RFLP)方法检测159例ATCI患者和151名健康正常对照者的IL-4C590T基因的单核苷酸多态性,用酶联免疫吸附(ELISA)法检测正常对照组的血清IL-4水平。结果正常对照组IL-4C590T基因TT型的血清IL-4水平[(31.85±5.68)pg/ml]显著高于TC型[(28.67±6.00)pg/ml]和CC型[(20.98±0.99)pg/ml],TC型又显著高于CC型(均P<0.01)。ATCI组与正常对照组IL-4C590T基因型和等位基因频率的分布差异无统计学意义(均P>0.05)。结论IL-4590T等位基因与高血清IL-4水平有关,IL-4C590T基因多态性与ATCI的发病无明显关系。Objective To explore the association between gene polymorphism of interleukin（IL）-4 C590T and serum level of IL-4 and atherothrombotic cerebral infarction （ ATCI）. （ Methods The gene polymorphisms of IL-4 C590T in 159 patients with ATCI and 151 normal control subjects were carried out by polymerase chain reaction （PCR）-restriction fragment length polymorphism （RFLP）. The serum level of IL-4 in normal control group was determined with enzyme linked immunosorbent assay （ELISA） method. Results In the normal control group, the serum level of IL-4 in TT genotype [ （ 31.85 ± 5.68 ） pg/ml ] was higher. significantly than those in TC [ （28.67 ± 6. 00） pg/ml] and Cc genotypes[ （20.98 ±0.99） pg/ml], TC genotype was higher significantly than CC genotype （ all P 〈0. 01 ）. The frequencies of genotypes and allele of IL-4 C590T were found no difference in statistics between ATCI group and normal control group（ all P 〉 0. 05）. Conclusions IL-4 590T allele is associated with higher level of serum IL-4. The C590T polymorphism is not associated with the incidence of ATCI.

关 键 词：动脉粥样硬化性血栓性脑梗死 白介素-4 基因多态性 

分 类 号：R743.3[医药卫生—神经病学与精神病学]