甘露聚糖酶3′序列缺失与酶功能间的关系初探  

Primary Study on Relation between Deleting 3′ Sequence of β-mannanase and Its Function

作　　者：王溪森[1] 刘正初[1] 李斌[1] 冯湘沅[1] 段盛文[1] 郑科[1] 成莉凤[1] 

机构地区：[1]中国农业科学院麻类研究所/农业部茎纤维生物质与工程微生物重点开放实验室,长沙410205

出　　处：《湖北农业科学》2009年第3期524-526,共3页Hubei Agricultural Sciences

基　　金：国家"863"计划项目(2006AA02Z249)

摘　　要：初步研究了3′端对甘露聚糖酶的功能重要性,即以甘露聚糖酶的二级结构为基础,用PCR扩增的方法获得3′端特异性缺失的甘露聚糖酶DNA片段,连接到载体上,表达并鉴定酶的活性。结果表明,删除390bp依然具有酶活性,3′端的部分序列不是酶具有功能所必需的。The paper studied the importance of 3＇sequence of β-mannanase.β-mannanase DNA were shorten by PCR according to its second structure. The DNA were linked to vector,and identified by clear halos of β-mannanase. 246bp was shorten from the 3＇sequence, but this could not make the enzyme loss its inactivity. Part sequence was not necessary for β-mannanase.

关 键 词：甘露聚糖酶 序列 删除 

分 类 号：Q939.129[生物学—微生物学] Q789