1，25二羟维生素D3联合塞莱昔布对乳腺癌细胞株Hs578T的增殖抑制及凋亡诱导作用  

The effects of 1,25(OH)_2D_3 associated with celecoxib on the proliferate inhibition and apoptosis induction in breast cancer cell line Hs578T

作　　者：汪勤[1] 孟刚[1] 鲍扬漪 孙昕[3] 何雪琦 

机构地区：[1]安徽医科大学病理解剖学教研室,合肥230032 [2]安徽医科大学第三附属医院肿瘤血液科,合肥230061 [3]安徽医科大学第一附属医院中心实验室,合肥230022

出　　处：《安徽医科大学学报》2009年第2期244-248,共5页Acta Universitatis Medicinalis Anhui

基　　金：合肥市科技局基金资助项目(编号:200715号)

摘　　要：目的研究1,25二羟维生素D3[1,25(OH)2D3](VitD3)联合塞莱昔布(CELE)对乳腺癌细胞株Hs578T的增殖抑制及凋亡诱导作用。方法采用四唑氮蓝比色(MTT)法检测细胞增殖,流式细胞仪测定细胞周期和凋亡率,末端脱氧核苷酸转移酶介导的原位酶标记(TUNEL)法计数凋亡细胞,免疫组化SP法分别检测凋亡与凋亡相关基因bcl-2、Bax表达的变化。结果10-7mol/L的VitD3联合5×10-5mol/L CELE、10-8mol/L的VitD3联合5×10-5mol/L CELE联合用药组通过MTT法测得72h对Hs578T细胞的A值分别为0.517±0.045、0.572±0.022,有统计学意义;流式细胞术显示72h后细胞凋亡实验组细胞凋亡率高于对照组凋亡率;细胞周期分析实验组可分别造成S期、G0/G1期阻滞;免疫组化SP法显示实验组凋亡相关基因Bax增高,bcl-2降低。结论VitD3联合CELE可以抑制Hs578T细胞增殖,改变细胞周期时相分布并诱导其调亡。Objective To study the effect of 1,25-dihydroxyvitamin D3 （ VitD3 ） associated with celecoxib （CELE） on growth and apoptosis in breast cancer cell line Hs578T. Methods MTT colorometric assay, flow cyometry and TUNEL were used to study the proliferation inhibition and apoptosis induction of Hs578T cells by VitD3 associated with CELE in vitro. The expression of bcl-2 and Bax were examined using immunohistochemistry S-P method. Results Incubation with VitD3 10-7 moL/L associated with CELE 5 × 10^-5 mol/L or VitD3 10^-8 mol/L associated with CELE 5 × 10^-5 mol/L, Hs578T cells exhibited significant growth inhibition. Flow cytometric analysis indicated cell＇s S or G0/G1 arrest along with increasing apoptotic peak and percentage. Treatment of Hs578T cells with VitD3 associated with CELE resulted in concentration-dependent decreases in bcl-2 and increased in Bax proteins. Conclusion VitD3 associated with CELE exhibit obvious inhibitory effects on the cell proliferation, cell cycle and induced apoptosis in Hs578T cells.

关 键 词：乳腺肿瘤 骨化三醇 

分 类 号：R737.9[医药卫生—肿瘤] R977.24[医药卫生—临床医学]