电镜在胃平滑肌母细胞瘤诊断中的作用  

Electron microscopic diagnosis of gastric leiomyoblastoma

作　　者：王文勇[1] 黄晓峰[2] 陈长生[3] 尹文[2] 王爽[2] 李永强[2] 戴晓汶[4] 钱斌[4] 

机构地区：[1]第四军医大学基础部病理学与病理生理学教研室,浙江杭州310013 [2]第四军医大学基础部中心实验室,陕西西安710033 [3]第四军医大学预防医学系卫生统计学教研室,陕西西安710033 [4]解放军第117医院病理科,浙江杭州310013

出　　处：《第四军医大学学报》2009年第8期730-732,共3页Journal of the Fourth Military Medical University

基　　金：陕西省自然科学基金(SJ08-ZT10);陕西省科技计划攻关项目(2008K04-02)

摘　　要：目的:研究电镜技术对胃平滑肌母细胞瘤的诊断价值.方法:外科手术或胃镜活检标本,大小约1mm×1mm×1mm,立即用30mL/L戊二醛固定,锇酸后固定.常规脱水、浸透,环氧树脂包埋.超薄切片后,用JEM-2000EX透射电镜进行细胞超微结构观察.结果:电镜下可见瘤细胞排列成片,偶尔可见细胞连接;多数瘤细胞胞质内可见明显肌丝和密斑、密体结构,粗面内质网呈囊状扩张、囊内见数量不等的中等电子密度蛋白分泌物,并可见粗面内质网池内隔离现象.核型多为圆形或卵圆形,部分不规则.结论:电镜下胃平滑肌母细胞瘤具有明确的肿瘤形态特征,对于该肿瘤具有确诊作用.通过电镜观察,还证明该肿瘤细胞空泡状的外观是由于粗面内质网扩张形成,而不是脂滴、糖原或黏液成分.AIM：To determine the role of electron microscopy in the diagnosis of gastric leiomyoblastoma. METHODS：The specimens from surgical or gastroscopy were 1 mm×1 mm×1 mm in size. The specimens were fixed by 30 mL/L glutaraldehyde immediately,post-fixed by osmic acid,dehydrated and permeated routinely,embedded by Epoxy resin,and observed with JEM-2000EX transmission electron microscope. RESULTS：The tumor cells arranged in patch and cell junction or junctional complex was seen occasionally between cells. Most of the neoplastic cytoplasms were filled with myofilaments,dense bodies and dense patches. Rough endoplasmic reticulum dilated as lake and a variety of protein secretion of intermediate electron density were found in the dilated cisternae. Intracisternal segregation was also observed. The nucleus were round or oval in shape and anomalistic nucleus were found in part of the cells. CONCLUSION：Electron microscopy shows the distinctive features of gastric leiomyoblastoma,which is very helpful for the diagnosis of gastric leiomyoblastoma. Electron microscopy also shows that the clear appearance of tumor cells is the result of the dilation of rough endoplasmic reticulum,not the fat droplets,glycogens or mucus in cytoplasm.

关 键 词：平滑肌瘤 上皮样 诊断 显微镜检查 电子 

分 类 号：R735[医药卫生—肿瘤]