黄荆子提取物EVn-50对人乳腺癌MCF-7细胞增殖和凋亡的影响  

Effect of Semen Viticis negundo extract EVn-50 on cell proliferation and apoptosis of human breast cancer cell line MCF-7

作　　者：肖集文[1] 庄英帜[1] 曹建国[2] 尹婵[1] 陈雪莲[1] 柳娜[1] 

机构地区：[1]南华大学附属第一医院肿瘤科,湖南衡阳421001 [2]南华大学肿瘤研究所,湖南衡阳421001

出　　处：《中华肿瘤防治杂志》2009年第3期175-178,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:研究黄荆子提取物EVn-50对人乳腺癌MCF-7细胞生长抑制和凋亡的诱导作用。方法:在体外培养人乳腺癌MCF-7细胞,采用表柔比星、他莫西芬和不同浓度的EVn-50处理MCF-7细胞,软琼脂和平皿克隆法测定EVn-50抑制MCF-7细胞增殖的量-效关系,TUNEL法、流式细胞术分析其细胞凋亡率和周期分布。结果:EVn-50对MCF-7细胞增殖的抑制作用随药物浓度的提高而更加明显,而在低浓度时却表现出轻微促细胞增殖现象(<10.0μg/mL),100.0μg/mL时抑制率为48.68%,实验组与对照组比较,差异有统计学意义,P<0.01。TUNEL法和流式细胞术分析结果表明,高浓度的EVn-50能诱导MCF-7细胞凋亡,阻滞细胞于G2/M期,降低细胞增殖指数,且也存在量-效关系。结论:EVn-50在高浓度时可抑制人乳腺癌MCF-7细胞增殖并诱导其凋亡,具有明显的抗肿瘤作用。OBJECTIVE： To investigate the effects of Semen Viticis negundo extract （EVn 50） on cell proliferation and apoptosis of human breast cancer cell line MCF-7. METHODS： Human breast cancer cell line MCF-7 was treated with ADM, tamoxifen and different con centration of Semen Viticis negundo extract （EVn-50）. The capacityactivity relationship of inhibit MCF-7 cell proliferation was detected by soft agar cloning and plate cloning formation assay. The rate of apop tosis and the distribution of cell cycle in MCF-7 cells were investigated by TUNEL and flow cytometry. RESULTS： Soft agar cloning and plate cloning formation assay showed that the proliferation of MCF-7 cells in vitro was inhibited by EVn-50 in concentration-dependent manners. But it could promote the proliferation in low concentration （〈10.0 μg/mL）. and it was 48. 68 % when the density of EVn-50 was 100.0 μg/mL. It was found that there was a significant difference between the experiment groups and control group, P〈0.01. TUNEL method and flow cytometry analysis showed that high concentrations of EVn-50 can induce apoptosis in MCF-7 cells, the cell block at the G2/M phase, the lower the index of cell proliferation, and also the volumeactivity relationship. CONCLUSION： The Semen Viticis ne gundo extract （EVn 50） has the effect of anti tumor for it can inhibit the proliferation and induce the cell apoptosis of MCF-7 cells.

关 键 词：中草药 乳腺肿瘤 细胞凋亡 

分 类 号：R737.9[医药卫生—肿瘤]