肺腺癌细胞中STAT3表达对细胞生长及药物敏感性的影响  

Expression of STAT3 and Its Effect on Cell Proliferation and Sensitivity to Cytotoxic Drugs in Lung Adenocarcinoma Cell

作　　者：余祖滨[1] 白莉[2] 闵家新[1] 肖颖彬[3] 白春学[4] 

机构地区：[1]第三军医大学附属新桥医院胸外科,重庆400037 [2]第三军医大学附属新桥医院全军呼吸内科研究所,重庆400037 [3]第三军医大学附属新桥医院心血管外科,重庆400037 [4]复旦大学附属中山医院呼吸科,上海200032

出　　处：《现代生物医学进展》2009年第8期1409-1412,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金(30700823)

摘　　要：目的:探讨STAT3表达变化对细胞生长及化疗药物敏感性的影响。方法:采用AG490处理细胞、SOCS3基因转染A549细胞后,Westernblot检测STAT3蛋白酪氨酸磷酸化水平变化;MTT法检测细胞增殖情况;不同浓度泰素处理细胞后观察细胞对药物的敏感性。结果:AG490处理细胞、SOCS3基因转染细胞后,Westernblot证实其能显著抑制STAT3蛋白酪氨酸磷酸化水平(P<0.01);MTT法结果示细胞增殖明显受到抑制;细胞对泰素敏感性显著增高。结论:STAT3能促进细胞增殖,AG490、SOCS3能显著抑制A549细胞中STAT3蛋白的活性,从而抑制A549细胞生长并增加其对化疗药物的敏感性。Objective： To explore the expression of STAT3 and its effect on cell proliferation and sensitivity to cytotoxic drugs. Methods： The A549 cells were treated by AG490 at 25μg/ml for 48h and stably transfected with SOCS3 by liposome. Tyrosine-phospho- rylated STAT3 level was measured by Western blot analysis. MTT assay were used to measure the cell proliferation and the sensitivity of cell to paclitaxel. Results： Tyrosine-phosphorylated STAT3 level decreased greatly in A549 cells treated by AG490 and stably transfected with SOCS3 （P〈0.01）. Growth of A549 cells showed significantly suppression, and subsequently increased the sensitivity to paclitaxel. Conclusion： AG490 and SOCS3 could inhibit the proliferation of A549 cells and enhance its sensitivity to paclitaxel by lowering the tyrosine-phosphorylated level of STAT3 protein which could improve the proliferation of cell.

关 键 词：STAT3 SOCS3 AG490 肺腺癌 

分 类 号：R734.2[医药卫生—肿瘤]