食管癌组织与外周血RASSF1A甲基化的检测及其临床意义  

RASSF1A methylation detection in esophageal cancer tissue and matched peripheral blood from the same patients

作　　者：秦豫培[1] 王立东[1] 常志伟[1] 郭涛[1] 李吉林 宋昕[1] 

机构地区：[1]河南省食管癌重点开放实验室郑州大学第一附属医院郑州大学基础医学院,河南郑州450052 [2]林州市姚村食管癌医院病理科,河南林州456592

出　　处：《中华肿瘤防治杂志》2009年第5期359-361,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：河南省医学科技攻关项目(20058);河南省食管癌重点开放实验室基金(20050227)

摘　　要：目的:通过对食管癌组织及同一患者对应的术前外周血中RASSF1A基因甲基化的检测,加深食管癌变分子机制的了解并为食管癌早期诊断和高危人群预警提供候选指标。方法:采用甲基化特异性PCR方法,分别检测来自食管癌高发区的30例食管癌患者血浆、肿瘤组织及癌旁正常组织中RASSF1A基因启动子甲基化状况。结果:食管癌组织RASSF1A甲基化阳性率为40%(12/30),而这12例癌组织甲基化阳性的患者其外周血甲基化阳性共7例,癌组织和外周血RASSF1A甲基化阳性一致率为58%(7/12),18例癌组织甲基化阴性的患者其外周血也均为阴性,阴性一致率为100%(18/18)。癌旁正常食管组织甲基化率为13%(4/30),明显低于癌组织(40%,12/30),P<0.05。7例外周血甲基化阳性的患者中,淋巴结转移阳性5例(55%,5/9),阴性2例(10%,2/21),两者差异有统计学意义,P<0.05。低分化鳞癌的RASSF1A甲基化阳性率(83%,5/6)明显高于中分化鳞癌(24%,5/21),P<0.05。结论:外周血RASSF1A甲基化可以反映同一个体食管鳞癌组织中RASSF1A基因甲基化的状态,可能是高危人群筛查重要候选分子标志之一。OBJECTIVE：To analyze RASSF1A methylation in esophageal cancer （EC） tissue and matched peripheral blood samples from the same patients, to further highlight the molecular mechanisms involved in EC and to provide a candidate target for high-risk subject screening and early diagnosis. METHODS： Methylation special PCR （MSP） was applied to determine RASSF1A methylation on esophageal cancer tissue, adjacent normal tissue and matched peripheral blood from the same patient at high incidence area in Henan Province. RESULTS： The detection rate of RASSF1A methylation in EC was 40% （12/30）, and of the 12 cases of positive RASSF1A methylation, 7 cases were found with RASSF1A methylation both in the cancer tissues and peripheral blood from the same patient, with a positive consistent rate of 58%（7/12）;18 cases were observed with negative methylation both in the cancer tissues and peripheral blood, with a consistant rate of 100 % （18/18）. RASSFIA methylation in EC was 40%（12/30）, which was much higher than that in the normal tissue （13%, 4/30）, and the difference was significant, P 〈 0. 05. In the 7 cases of RASSF1A methylation in peripheral blood, 5 cases were with lymph node metastasis （ 55 %, 5 / 9）, Two cases were without lymph node metastasis 10%（2/21）, and the difference was significant, P〈0. 05. RASSF1A methylation rate was higher in poorly differentiaed EC （83%,5/6） than well differentiated EC （24%, 5/21）, P〈0.05. CONCLUSION： RASSF1A methylation in peripheral blood can partly represent the similar changes in cancer tissue from the same patient, and may be a promising biomarker for high risk subject screening.

关 键 词：食管肿瘤 RASSF1A 甲基化 外周血 甲基化特异性PCR 

分 类 号：R735.1[医药卫生—肿瘤]