抗氧化蛋白酶  

Advance in study of oxidatively stable protease(OSP)

作　　者：王伟[1] 孙谧[1] 郝建华[1] 

机构地区：[1]中国水产科学研究院黄海水产研究所海洋产物资源与酶工程室,青岛266071

出　　处：《生命的化学》2009年第2期223-226,共4页Chemistry of Life

摘　　要：抗氧化蛋白酶在洗涤剂工业有重要应用价值。天然环境中可以筛选到产抗氧化蛋白酶的菌株,但分析抗氧化酶的基因无法找到抗氧化规律。定点突变法可以增强普通洗涤蛋白酶的抗氧化性,但活性降低。抗氧化蛋白酶空间结构的研究结果,尚未能阐明抗氧化机理及氧化后底物专一性改变的原因。研究抗氧化蛋白酶对酶制剂的研发有重要指导意义。Oxidatively stable proteases （OSP） are of important value as detergent additives. Many OSP-producing strains are screened from the environment, but the number of the OSP genes is still scanty and the alignment of the present eight OSPs does not demonstrate the common characters amongst the amino acid sequences. Oxidation-resistant detergent protease mutants can be obtained at the cost of the activities by using site-directed mutagenesis technique. Although it is inferred that the slow oxidation of Met in the vicinity of the catalytic Set is a possible mechanism underlying the oxidative stability of KP-43, the detailed mechanism is still puzzling. Perspective studies are required to investigate the theoretical problems on OSP which will bring further insights of the relationship between the protease structure and function. The results will provide clues to research and develop detergent products of OSP.

关 键 词：抗氧化蛋白酶 筛选 蛋白质工程 空间结构 

分 类 号：Q55[生物学—生物化学]