香附子内生菌的分离鉴定及活性检测  

The Isolation、Identification and Activity Detection of Endogenous Fungus from Cyperus rotundus L.

作　　者：王娜娜[1] 秦宝福[1] 刘建党[1] 史鹏[1] 常佳丽[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《中国农学通报》2009年第8期46-49,共4页Chinese Agricultural Science Bulletin

基　　金：西北农林科技大学科研专项基金(08080257)

摘　　要：从药用植物香附子中分离出4种内生菌,经初步鉴定分别归属于曲霉属(代号为XFf06.1、XFf06.2),固氮菌属(XFb)和链霉菌属(XFa)。对这些内生菌发酵产物提取液活性进行测定,结果表明:其发酵产物均有稳定的抑菌活性,其中链霉菌XFa对枯草芽孢杆菌的抑菌率特别强,XFf06.1菌的发酵液提取物对苹果腐烂病原菌的抑菌率高达96.3%。4 strains of Entophytes were isolated from Cyperus rotundus L. in this study, which were respectively belonged to AspergiUus （named XFf36.1 and XFf36.2）, Azotobacter Beijerinck （XFb） and Streptomyces （XFo）. The result of detecting its antimicrobial activity showed that the production of the fermentative culture of the screened strains presented certain stable antimicrobial activity, especially XFa appeared highly antimicrobial activity to Bacillus subtilis and the antimicrobial activity of XFf6.1 to Valsa mali miyabe et. Reached 96.3%.

关 键 词：香附子 内生菌 分离鉴定 抗菌活性 

分 类 号：Q93-3[生物学—微生物学]