ETH、KT和6-BA对绿豆幼苗形态建成和生化成分的效应研究  

Effects of Ethylene, Kinetin and 6-BA on the Morphological and Biochemical Composition of Mung Bean Seedlings

作　　者：康玉凡[1] 谷瑞娟[1] 王保民[1] 廖永霞[1] 肖伶俐[1] 罗珊[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100094

出　　处：《中国农学通报》2009年第9期19-25,共7页Chinese Agricultural Science Bulletin

基　　金：宁波市农业科技攻关项目"豆芽烂芽成因极其预防技术研究"(2007C10078);国家食用豆现代产业技术体系项目资助

摘　　要：研究了ETH、KT、6-BA及ETH—KT、ETH—6-BA复配处理对绿豆幼苗形态、生化成分、内源激素及下胚轴细胞组织结构的调节效应,为绿豆芽菜的安全生产提供理论和技术支持。设置上述5个激素处理及对照,采用人工气候箱技术培育绿豆芽,并进行各项指标测定。结果显示:与对照相比,处理组均可使绿豆幼苗下胚轴缩短和增粗、主根长缩短、侧根数减少(P<0.05),其调节效应大小为ETH—6-BA>ETH—KT>ETH>6-BA>KT;ETH—6-BA可使下胚轴缩短35.78%,增粗26.51%,主根缩短65.43%,并完全抑制侧根的产生。不同处理对绿豆幼苗的生化成分的效应大小存在差异(P<0.05),ETH可使绿豆幼苗可溶性蛋白、可溶性糖和维生素C含量分别增加25.1%、66.07%和163.9%;各处理调节效应的大小依次为ETH>ETH—KT>ETH—6-BA。各处理均在一定程度上改变了绿豆幼苗的内源激素含量,6-BA及ETH—6-BA可使IAA含量分别降低88.9%和33.3%,使ZR含量分别降低35.3%和22.8%,ETH—6-BA处理可使ABA含量增加156.9%。对绿豆幼苗下胚轴的增粗作用,ETH增加了髓内细胞数目和单个皮层细胞面积,减少了皮层细胞数,表现为髓和形成层面积的增大;6-BA与ETH—6-BA增加了髓和皮层的单个细胞面积,同时增大了髓、形成层和皮层的面积。表明ETH、KT、6-BA及其复配对绿豆芽苗的生长均有不同程度的调解效应,而其各自的调节方式又有所不同。In order to provide the support of theory and technology for mung bean sproui production, the effects of ethylene （ETH）, kinetin （KT）, 6-BA and their compound ETH--KT, ETH---6-BA on the morphological features, biochemical composition, endogenous hormones and tissue structure of mung bean seedlings were investigated. By the growth chamber method, five hormone treatments mentioned above and a control were set during mung bean seeding cultivating. Compared to the control, the number of lateral roots was reduced, the growth of roots and hypocotyls were suppressed and the diameter of hypocotyls was increased four five treatments （P〈0.05）. Their regulation effects were ETH--6-BA〉ETH--KT〉ETH〉6-BA〉KT respectively. The length of hypoeotyls was suppressed at 35.78%, the diameter of hypocotyls was increased at 26.51%, the length of root was suppressed at 65.43% and the secondary roots were inhibited completely by ETH--6-BA respectively. The biochemical composition and endogenous hormones in mung bean seedlings were changed for five treatments （P〈0.05）. The regulation effects were ETH〉ETH--KT〉ETH--6-BA respectively. The soluble protein content was increased at 25.1%, the soluble sugar content was increased at 66.07%, and Vitamin C content was increased at 163.9% by ETH. The endogenous hormones in mung bean seedlings were changed for five treatments. The indole-3-acetic acid （IAA） content were decreased at 88.8 % and 33.3%, while ZR content were decreased at 35.3%, 22.8% by 6-BA and ETH--6-BA respectively. ABA content was improved at 156.9% by ETH--6-BA. The thickened effect of ETH on the hypocotyls diameter was explained by increasing number of pith cells and decreasing area of single cortex cell, showed the area of the marrow and cambium increased. Theirs effects of 6-BA and ETH---6-BA was by increasing the area of single pith ceils and cortex cells, and increasing the area all of marrow, cambium and the cortical area. Ethylene （ETH）, kinetin （KT）, 6-BA and their compound have dif

关 键 词：ETH KT 6-BA 绿豆幼苗 形态特征 生化成分 内源激素 组织结构 

分 类 号：S311[农业科学—作物栽培与耕作技术]