自配试剂在COULTER流式细胞仪检测NK细胞和B细胞中的应用  

Application of Self-made Reagent in the Detection of NK Cells and B Cells by Beckman-Coulter Flow Cytometer

作　　者：徐明辉[1] 陈智平[2] 秦雪[1] 莫武宁[1] 王立秋[3] 李若林[1] 林发全[1] 黄军强[1] 李山[1] 

机构地区：[1]广西医科大学第一附属医院临床实验部,广西医科大学医学检验系,广西南宁530021 [2]广西医科大学公共卫生学院,广西南宁530021 [3]哈尔滨医科大学第五附属医院检验科,黑龙江大庆163316

出　　处：《标记免疫分析与临床》2009年第2期103-105,共3页Labeled Immunoassays and Clinical Medicine

摘　　要：探讨美国COULTER流式细胞仪试剂国产化问题。将自配全血溶解试剂、鞘液及清洗液的理化性质、精密度、准确度、稳定性与原装试剂进行对比分析。分析结果显示自配试剂与原装试剂理化性质一致,采用自配试剂测定20份病人标本NK细胞和B细胞并与原装试剂对比,差异无统计学意义(P>0.05),两者呈高度正相关r>0.95。自配试剂稳定性好,放置1年后,与原装试剂分别测定15份标本,结果差异无统计学意义(P>0.05),两者呈高度正相关r>0.91,说明自配试剂完全可以代替美国COULTER流式细胞仪原装进口试剂进行NK细胞和B细胞的检测。To explore the localization issues of reagents used in Beckman-Coulter Flow Cytometer, the self-made haemolysis reagent, sheath liquid and cleansingsolution were used to detect NK cells and B cells in 20 patients samples by Beckman-Coulter Flow Cytometer, their physicochemical properties, precisions, accuracy and stability were compared with import reagents. The results showed that the physicoehemical properties of self-made reagents were those of import reagents. There were no significantly difference between self-made and import reagents in the detection of NK cells and B cells （P 〉 0.05 ）. There was a highly positive correlation between them （ r 〉 0.95 ）. Self-made reagent showed a very good stability within one year. The results indicate that self-made reagent for Beckman-Coulter Flow Cytometer can completely replace import reagents in the detection of NK cells and B cells.

关 键 词：流式细胞仪 试剂研制 NK细胞 B细胞 

分 类 号：R446.6[医药卫生—诊断学]