亚砷酸诱导人低分化鼻咽癌细胞系肿瘤抑制基因表达  

Up-regulation of the tumor-suppressing gene expression in nasopharyngeal carcinoma cells induced by Arsenic Trioxide

作　　者：梁海慧 张月飞[2] 姚俊[2] 

机构地区：[1]珠海市第二人民医院耳鼻咽喉科,广东珠海519020 [2]广东医学院附属医院耳鼻咽喉头颈外科,广东湛江524001

出　　处：《中国耳鼻咽喉头颈外科》2009年第4期179-182,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：广东省科技计划项目(2006B36001004)

摘　　要：目的探讨亚砷酸对人低分化鼻咽癌细胞系CNE-2Z的作用及其可能机制,进一步认识亚砷酸的抗肿瘤作用。方法体外培养的CNE-2Z细胞分别加入不同浓度的亚砷酸,作用不同时间。台盼蓝染色法筛选出亚砷酸抑制CNE-2Z细胞生长的最佳浓度,检测IC50值;流式细胞术检测细胞周期的改变;同时应用逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)检测p16mRNA、Ras相关结构域家族1A基因(ras association domain family 1A,RASSF1A)mRNA和DNA甲基转移酶3B(DNA methyltransferase,DNMT3B)mRNA表达。结果亚砷酸对细胞增殖的抑制作用具有明显的时间和剂量效应,不同浓度亚砷酸作用于细胞24、48、72小时后,IC50值分别为(1.50±0.05)、(1.09±0.13)、(0.65±0.04)μmol/L。亚砷酸使细胞周期阻滞于S期和G2/M期,并能上调RASSF1A mRNA的表达,但对p16mRNA表达没有明显影响。结论亚砷酸能够抑制CNE-2Z细胞增殖,其可能机制是通过诱导抑癌基因RASSF1A表达上调,恢复其活性,抑制CNE-2Z增殖。OBJECTIVE This study is to investigate the effects on nasopharyngeal carcinoma cell lines （CNE-2Z） induced by As2O3 and its possible mechanisms. METHODS CNE-2Z cells were treated with various concentrations of As2O3 for different times. The IC50 values were detected by trypan blue stain assay to choose the optimal concentration of As2O3. Cell cycle redistribution was analyzed by flow cytometry. The expression levels of p16. RASSF1A. DNMT 3B mRNA were assessed by RTPCR. RESULTS The suppression of cell proliferation by As2O3 was time and dose-dependent. After being treated with different concentration of As2O3, the IC50 values of As2O3 were （1.50±0.05） , （1.09±0.13） , （0.65±0.04） μmol/L at 24, 48, and 72h, respectively. As2O3 also arrested CNE-2Z cells in G2/M phase of cell cycle. After CNE-2Z cells being treated with As2O3, the expression of RASSF1A mRNA were up-regulated （P〈0.01） , but there wasn＇t effect on the expression of p16 mRNA. CONCLUSION As2O3 can inhibit CNE- 2Z cells proliferation. It may be one of the mechanisms that As2O3 could up-regulate the expression of RASSF1A mRNA, restore their activities, and inhibit the proliferation.

关 键 词：亚砷酸盐类 鼻咽肿瘤 基因 肿瘤抑制 

分 类 号：R739.65[医药卫生—肿瘤]