菌斑生物膜原位干预模型的建立  

Study on intervention models of plaque biofilm in situ

作　　者：鲁纯[1] 胡凡[2] 徐艳[1] 李谨[1] 王娟[1] 倪杰[1] 覃文[1] 陈武[1] 

机构地区：[1]南京医科大学口腔医学研究所,南京医科大学附属口腔医院牙周科,南京210029 [2]南京医科大学医学测试中心,南京210029

出　　处：《口腔医学》2009年第4期174-176,共3页Stomatology

基　　金：江苏省高校自然科学基础研究基金项目(06KJB320073)

摘　　要：目的通过对原位菌斑生物膜发育过程及早期成熟菌斑生物膜的观察,探讨所建立的菌斑生物膜模型用于菌斑化学干预研究的可能性。方法建立原位菌斑生物膜模型,运用激光共聚焦扫描显微镜结合荧光染色技术观察原位菌斑生物膜0～48h的发育过程及48h菌斑生物膜的活性和厚度。结果可观察到0～48h菌斑生物膜从无到形成到成熟,细菌排列趋于密集,菌斑厚度逐渐增加的过程。48h的菌斑生物膜活性和厚度可检测。结论建立的菌斑生物膜模型可观察原位菌斑的形成过程、形态及活性,适用于菌斑化学干预的研究。Objectives To explore whether the established model of dental plaque biofilm can be used in the research of chemical intervention on plaque biofilm via observing the development of biofilm and the vitality of earlier mature biofilm plaque. Methods An in vivo biofilm model was established. The development of biofilms in 48 hours in vivo was observed and the vitality and thickness of earlier mature biofilms were measured by confocal laser scanning microscope and the vital fluorescence techniques. Results The development of biofilms in 48 hours in vivo was observed and the vitality and thickness of earlier mature biofilms were measured. Conclusion The established biofilm model can be used in the research of chemical intervention on plaque biofilm in situ.

关 键 词：菌斑生物膜 化学干预 模型 激光共聚焦扫描显微镜 

分 类 号：R781.49[医药卫生—口腔医学]