约氏不动杆菌(Acinetobacter johnsonii)的分离及其聚磷特性的研究  

Study on Isolation and Screening of Acinetobacter johnsonii and Characteristics of Phosphorus Removal

作　　者：连丽丽[1] 姜华[1] 朱昌雄[2] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116029 [2]中国农业科学院农业环境与可持续发展研究所,北京100081

出　　处：《辽宁农业科学》2009年第2期18-21,共4页Liaoning Agricultural Sciences

基　　金："十一五"国家水体污染控制与治理科技重大专项项目(2008ZX07425);辽宁省教育厅项目(2008S135)

摘　　要：采用定性、定量相结合的方法，从城市污水中筛选到一株高效聚磷菌（CH-1），经生理生化特征及16 rDNA序列分析，鉴定该菌株为不动杆菌属的约氏不动杆菌（Acinetobacter johnsonii）。进行了该菌株的生长曲线、含磷培养液的pH、磷酸盐浓度变化及去磷效果等测定。结果表明：该菌在培养6—8h时繁殖量达到最大（0．54mg／L），培养液pH值达到最高（8．88），而磷酸盐浓度降至最低（1．08mg／L）。好氧条件下，含磷培养液培养72h后，培养液中磷浓度由10mg／L降至3．476mg／L，去磷率高达65．24％。CH-1菌处理模拟废水的除磷率高达68．88％，具有较强的聚磷能力。A bacterial strain CH-1 with high capability of accumulating poly-P was isolated from municipal sewage and identified as Acinetobacter johnsonii by using qualitative and quantitative screening methods. Some important factors, OD, pH value, phosphorus concentration and phosphorus removal ability of CH-1, were studied to evaluate their change by time. Results showed that OD and pH value kept increasing, and phosphorus concentration kept decreasing by time. After 6 - 8 h, OD and pH value reached the highest, 0.54 and 8.88 respectively, and phosphorus concentration was below 1.08 mg/L. In aerobic condition, phosphorus concentration in the medium declined from 10 mg/L to 3. 476 mg/L, phosphorus removal rate reached 65.24% after 72 h. The phosphorus concentration in CH-1 reached 11.25 % of its weight, indicating that CH-1 has a high capability of accumulating phosphate. The bacterial strain treated with synthetic wastewater for 72 h, phosphorus removal rate reached 68.88%.

关 键 词：聚磷菌 筛选 聚磷特性 

分 类 号：X172[环境科学与工程—环境科学]