一株野鸭源H4N6亚型禽流感病毒A/mallard/Yancheng/2005的全基因组克隆和序列分析  

Cloning and Genetic Analysis of the Entire Genome of A/mallard/Yancheng/2005(H4N6) Avian Influenza Virus

作　　者：彭宜[1] 薛峰[1,2] 李彦芳[1] 陈浩 彭大新[1] 刘秀梵[1] 

机构地区：[1]扬州大学农业部畜禽传染病学重点开放实验室,扬州225009 [2]江苏出入境检验检疫局,南京210001 [3]盐城国家级珍禽自然保护区管理处,盐城224000

出　　处：《畜牧兽医学报》2009年第4期548-553,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：十一五国家支撑计划项目(2006BAD06A01);盐城市科技发展计划(YK2007047)

摘　　要：应用流感病毒通用引物对盐城珍禽自然保护区野鸭泄殖腔棉拭子分离株禽流感病毒A/mallard/Yancheng/2005(简称Mallard/YC/2005)(H4N6)进行全基因组序列扩增,结合GenBank中的相关序列进行遗传进化分析。结果表明,Mallard/YC/2005(H4N6)HA基因与A/duck/Siberia/1701/1996(H4N6)的核苷酸同源性最高(97.5%),推导的氨基酸剪切位点序列为PEKASR,为典型低致病性禽流感病毒的特征序列;神经氨酸酶(NA)、非结构蛋白(NS)均没有氨基酸缺失;基质蛋白2(M2)与宿主特异性有关的位点及碱性聚合酶2(PB2)的627位都是亲禽类细胞的氨基酸;M基因与家鸭分离株A/duck/Yangzhou/02/2005(H8N4)的核苷酸同源性为99.4%,PA基因与家鸭分离株A/duck/Jiangxi/1286/2005(H5N2)的核苷酸同源性达98.9%,说明这2个基因已经在家鸭体内存在并参与了基因重排。An H4N6 subtype avain influenza virus（AIV）,A/mallard/Yancheng/2005（H4N6）,was isolated from cloacal swab of mallard in the national protection grade in Yancheng country.The eight full-length genes,including the 5′and 3′ends of virus were amplified by using the universal primers.Amino acid sequence comparisons and phylogenetic analysis of A/mallard/Yancheng/2005（H4N6）AIV was made with other sequences available in the Influenza Sequence Database.The results showed that the motif of HA cleavage site is PEKASR,which was the characteristics of low pathogenic AIV.Both the NA and NS gene had no residue lost.The amino acid residues related to host specialty of matrix protein 2 were all avain specialty,and the 627 residue of PB2 was Glutamic acid（E）.The nucleotide homology of M gene with A/duck/Yangzhou/02/2005（H8N4） AIV was 99.4%,the PA gene had genetically close relationships with that of A/duck/Jiangxi/1286/2005（H5N2） AIV,these results suggest that both M and PA gene had circulated in the domestic duck and involved in gene reassortment.

关 键 词：Mallard/YC/2005(H4N6) 禽流感病毒 全基因组序列 序列分析 

分 类 号：S852.659.5[农业科学—基础兽医学]