小麦导入磷酸烯醇式丙酮酸羧化酶(PEPCase)基因的初步研究  

The Preliminary Research of Transferring PEPCase Gene into Wheat

作　　者：张彬[1] 马建军 贾栋[3] 石云鹭[4] 高志强[3] 丁在松[5] 

机构地区：[1]山西农业大学园艺学院,山西太谷030801 [2]山西省平鲁区职业中学,山西平鲁036800 [3]山西农业大学农学院,山西太谷030801 [4]中国农业大学农学与生物技术学院,北京100094 [5]中国农业科学院作物科学研究所,北京100081

出　　处：《安徽农业科学》2009年第11期4900-4901,共2页Journal of Anhui Agricultural Sciences

基　　金：山西农业大学科技创新基金(2008017)

摘　　要：[目的]将磷酸烯醇式丙酮酸羧化酶(PEPCase)基因导入普通小麦临优145。[方法]利用根癌农杆菌遗传转化系统,以普通小麦临优145为材料,将磷酸烯醇式丙酮酸羧化酶(PEPCase)基因,导入小麦胚性愈伤组织中,用含潮霉素(hyg)的筛选培养基连续筛选,并从分子水平上检测该基因。[结果]获得了hyg抗性植株,经GUS组织化学染色检测表明,抗性苗叶片被染成了深蓝色;PCR检测出目标条带。[结论]初步证明磷酸烯醇式丙酮酸羧化酶(PEPCase)基因已经导入受体材料中。[Objective] The research aimed to transfer the PEPCase gene into wheat Linyou145.[Method] The PEPCase gene was transformed to embryonic callus in wheat Linyou145 by Agrobacterium tumefaciens-mediated transformation.Hygomycin Resistant（hyg） plants were obtained by screening in selective medium containing Hygomycin.The gene was detected on the lever of molecular.[Result] By means of PCR amplification and GUS histochemical analysis of Hyg-resistant plants showed that the target gene had been integrated into wheat accompany with hpt gene.Blue spots were observed in resistant leaves.[Conclusion] Its preliminary testified PEPCase gene had been transformed to wheat Linyou145.

关 键 词：小麦 磷酸烯醇式丙酮酸羧化酶(PEPCase)基因 农杆菌介导法 抗性植株 

分 类 号：S512.1[农业科学—作物学]