拟康氏木霉和白腐菌混菌发酵处理稻草秸秆的研究  

Study on degradation of straw stalk by co-fermentation of Trichoderma pseudokoningii and Phanerochaete chrysosporium

作　　者：王志[1] 陈雄[1] 王实玉[1] 王永泽[1] 李冬生[1] 

机构地区：[1]湖北工业大学生物工程学院湖北省工业微生物重点实验室,湖北武汉430068

出　　处：《可再生能源》2009年第2期36-39,共4页Renewable Energy Resources

基　　金：湖北省教育厅重点项目(D20081401);武汉市科技局重点项目(200820212029)

摘　　要：以拟康氏木霉3.3002(Trichoderma pseudokoningii)和白腐菌5.776(Phanerochaete chrysosporium)的混合菌发酵处理稻草秸秆,并对处理结果进行了试验研究。研究结果表明,当接入拟康氏木霉与白腐菌时间间隔为3d,接种液体积比为2ml∶2ml,氮源为0.3%(质量分数)的(NH4)2SO4,发酵时间为8d时,稻草秸秆纤维素降解率和木质素降解率分别为40.38%和31.52%,比单独使用拟康氏木霉(纤维素和木质素降解率分别为24.62%,13.78%)或白腐菌(纤维素和木质素降解率分别为6.96%,20.06%)处理稻草秸秆的效果有显著提高。Degradation of straw stalk was investigated by co-fermentation of Trichoderma pseudokoningii and Phanerochaete chrysosporium. The result showed that 40.38% of cellulose and 31.52% of lignin was decomposed at following conditions： inoculating Phanerochaete chrysosporium first, after 3 days, inoculating Trichoderma pseudokoningii at inoculation volume ratio of 2 ml：2 ml, 0.3% of （NH4）2SO4 in mass fraction was chosen as the nitrogen source, fermentation duration is 8 days. The degradation of cellulose and lignin by using mixed inoculums was much higher than that by using single inoculums （24.62% ,13.78% for Trichoderma pseudokoningii, 6.96% ,20.06% for Phanerochaete chrysosporium）.

关 键 词：混合菌发酵 稻草秸秆 纤维素 木质素 

分 类 号：TK6[动力工程及工程热物理—生物能] Q939.97[生物学—微生物学]