不同转移能力肺癌细胞株差异非编码RNA的鉴定  

Identification of the non-coding RNA in the cell line derived from lung cancer with different transferring ability

作　　者：朱彦君[1] 夏伟[2] 曹国军[2] 丁红梅[2] 高亚萍[2] 杨光[2] 邵宁生[2] 

机构地区：[1]中国人民解放军空军总医院胸外科,北京100142 [2]中国人民解放军军事医学科学院基础医学研究所,北京100850

出　　处：《中华肿瘤防治杂志》2009年第4期246-248,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家973课题项目(2005CB724600)

摘　　要：目的:探讨不同转移能力的肺癌细胞株中miRNA表达差异,并分析miRNA在肺癌转移过程中的功能及意义。方法:利用非编码小RNA芯片技术,检测高转移肺癌细胞株(PGBE1)与低转移肺癌细胞株(PGLH7)间miRNA的表达差异,并通过qPCR鉴定。结果:非编码小RNA芯片分析显示,在不同转移能力肺癌细胞中得到4条差异表达的miRNA,分别为hsa-mir-34b、hsa-mir-23a、hsa-mir-23b和hsa-mir-199a-5p。实时定量PCR对差异的miRNA进行鉴定,4条差异的miRNA在低转移细胞株(PGLH7)中的丰度均高于其在高转移细胞株(PGBE1)中的丰度,P<0.05,与芯片结果吻合。结论:4条miRNA可能与肺癌转移相关,对miRNA功能探讨将有助于研究肺癌转移的分子机制。OBJECTIVE： To identify the specific expression profile of microRNA （miRNA） in the different cell line （PGBE1 and PGLH7） and discuss the potential biological function of the specific miRNAs. METHODS： the expression profile of miRNA in the different transferring cell lines were checked by the non coding RNA chip. The specific expressed miRNAs were identified by quantitive PCR. RESULTS： Four differentially expressed miRNA （hsa-mir-34b, hsa-mir-23a, hsa-mir-23b and hsa-mir-199a-5p） were indentified by quantitive PCR. The expression level in PGLH7 was higher than the level in PGBE1, P 〈 0.05. CONCLUSION： Four miRNAs were identified to related with the transferring ability of lung cancer.

关 键 词：肺肿瘤 RNA 基因表达调控 肿瘤转移 

分 类 号：R734.2[医药卫生—肿瘤]