反义寡核苷酸对骨肉瘤细胞MTA1基因表达及侵袭性影响的研究  

Effects of MAT1 antisense oligodeoxynucleotide on expression in human osteosarcoma cell line MG-63 and ability of invasion and metastasis

作　　者：曾恒[1] 陈安民[1] 李锋[1] 杨彩虹[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院矫形外科,湖北武汉430030

出　　处：《中华肿瘤防治杂志》2009年第4期284-287,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:合成人肿瘤转移相关基因(MTA1)的反义脱氧寡核苷酸,观察其转染后对人骨肉瘤MG-63细胞系中MTA1表达及骨肉瘤侵袭性的影响。方法:免疫细胞染色观察人工合成正义、反义及无意义MTA1基因片段转染人骨肉瘤细胞MG-63后,人肿瘤转移相关基因的表达;RT-PCR和蛋白质印迹法检测MTA1基因mRNA和蛋白质表达水平的变化;Boyden小室体外侵袭实验检测转染前后细胞侵袭力的变化。结果:反义寡核苷酸处理骨肉瘤细胞后,MTA1 mRNA的表达下降(吸光度之比为25.09±0.21),与正义链组、无意义链组和空白对照组(35.19±0.17、33.20±0.23和37.17±0.18)相比差异有统计学意义,P<0.05。Boy-den小室体外侵袭实验检测显示,反义寡核苷酸处理后骨肉瘤细胞的透膜能力明显下降。结论:MTA1同骨肉瘤的转移能力密切相关,反义寡核苷酸的转染可阻遏骨肉瘤细胞中MTA1的表达,并因此有可能限制骨肉瘤的侵袭和转移。OBJECTIVE： To study the effect of metastasis-associated-1 phosporothioated antisense oligodeoxynucleotide on MAT 1 expression and the ability of invasion and metastasis in human osteosarcoma cell line MG-63. METHODS： The antisense, sense and nonsense oligodeoxynucleotides for MTA 1 gene were synthesized and delivered into the human osteosarcoma cell line MG-63. The effects of oligodeoxynucleotides were examined with immunobistochemistry, RT-PCR and Western blot. The abilities of invasion and matastasis were detected by Boyden chamber invasive models. RESULTS： The expression of MTA1（25.09±0.21 ） was significantly decreased after the cells were treated with antisense oligodeoxynucleotide compared with the control cells （35. 19±0. 17, 33.20±0.23 and 37.17±0.18）, so as to the abilities of invasion and metastasis,P〈0.05. CONCLUSIONS： There may be a relationship between MTA1 and invasive potentiality of human osteosarcoma cells. The MTA1 gene antisense oligodeoxynucleotide may significantly inhibit the expression of MTA1 and abilities of invasion and metastasis in MG-63 cells.

关 键 词：骨肉瘤/遗传学 寡核苷酸类 反义 细胞 培养的 肿瘤浸润 

分 类 号：R738.1[医药卫生—肿瘤]