长春花种质资源遗传多样性的ISSR分析  

ISSR Analysis of Genetic Relationships between Forty Varieties of Catharanthus roseus

作　　者：姚丹青[1] 潘彬也[1] 热娜.多里坤 关媛[1] 潘俊松[1] 何欢乐[1] 唐克轩[1] 蔡润[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240

出　　处：《上海交通大学学报（农业科学版）》2009年第2期138-142,共5页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：上海市科技兴农项目[沪农科攻字(2005)第1-1-5号];上海交通大学PRP项目(T15012042)

摘　　要：本研究利用ISSR分子标记技术对40个长春花种质资源进行了遗传关系分析,多态性PCR扩增结果表明:在115个位点,其中多态位点78个,多态位点百分率为67.8%,多态性较高;POPGENE32软件计算结果表明,40个长春花品种平均有效等位基因数为1.6384,Nei遗传多样性指数平均为0.3735,平均Shannon信息指数为0.5546;应用NCSYS软件计算得到品种间的遗传相似系数介于0.150～0.900,平均为0.584。通过聚类分析将这40个长春花种质分成7组,该聚类结果与以前根据形态进行的分类结果有极大的相似性。Genetic relationships between forty varieties of Catharanthus roseus which are suitably cultivated in Shanghai were analyzed by ISSR （inter-simple sequence repeat） markers. Genetic diversity and similarity were analyzed in molecular level and the purpose of this study is further perfecting the evaluation of species resource in Catharanthus roseus. The result showed that a total of 115 bands based on 20 primers were detected, out of which 78 were polymorphic （67.8%）. As analyzed by POPGENE32, the average value of effective number of alleles, Nei＇s gene diversity and Shannon＇s information index were 1.6384, 0.3735, 0.5546, respectively. As analyzed by NCSYS, the Nei＇s genetic similarity coefficient between cuhivars ranged from 0.150 to 0.900, mean is 0.584. Clustering analysis showed that the 40 varieties of C. roseus could be distinctively classified into 7 groups. This is in accord with previous result made by morphological study.

关 键 词：长春花 遗传多样性 遗传相似性 ISSR 聚类分析 

分 类 号：S685.99[农业科学—观赏园艺]