雌激素及其阻滞剂对Ishikawa细胞中ETS-1和VEGF调控的研究  

The study of regulation of ETS-1 and VEGF by estrogen and estrogen receptor blocker in endometrial carcinoma cell line Ishikawa

作　　者：张靖[1] 汪宏波[1] 王泽华[1] 王小萼[1] 徐亚辉[1] 徐媛[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院妇产科,武汉430022

出　　处：《现代妇产科进展》2009年第4期245-248,共4页Progress in Obstetrics and Gynecology

摘　　要：目的:探讨雌激素及其阻滞剂对子宫内膜癌细胞系Ishikawa细胞中转录因子ETS-1和血管内皮细胞生长因子(VEGF)表达的调控机制。方法:用RT-PCR法检测分别使用不同浓度雌激素、雌激素受体阻滞剂氟维司群处理后Ishikawa细胞中ETS-1基因表达水平的变化;用细胞爬片免疫组化法检测分别使用这两种不同浓度药物后Ishikawa细胞中ETS-1与VEGF表达的差别;采用ELISA法检测分别使用两种不同浓度的药物后Ishikawa细胞培养液中VEGF蛋白表达量的变化。结果:(1)随着雌激素浓度增加,ETS-1基因表达上调(FP=3.84,P<0.05);随着氟维司群浓度增加,ETS-1基因表达下调(FP=7.01,P<0.01);(2)随着雌激素浓度增加,ETS-1(FP=3.72,P<0.05)和VEGF(FP=3.68,P<0.05)蛋白质水平表达都增加;随着氟维司群浓度增加,ETS-1(FP=4.37,P<0.05)和VEGF(FP=4.09,P<0.05)蛋白质水平的表达都减少;(3)随着雌激素浓度增加,分泌型VEGF蛋白表达量上升(FP=3.91,P<0.05);随着氟维司群浓度增加,分泌型VEGF蛋白表达量下调(FP=7.34,P<0.01)。结论:雌激素可促进Ishikawa细胞中ETS-1和VEGF的表达,VEGF表达与ETS-1表达呈正相关。Objective：To explore the role of E2 and it＇s receptor blocker in the regulations of ETS-1 and VEGF genes expression in ER-positive human endometrial carcinoma cell line Ishikawa. Methods：ETS-1 gene expression in Ishikawa cells was detected by RT-PCR after the treatments of either estrogen or fulvestrant in different concentrations. The expression differenees of ETS-1 and VEGF in Ishikawa cells were detected by immunohistoehemieal technique following the same treatment. Moreover, the secretion of VEGF protein in the culture media of Ishikawa cells was also detected by ELISA. Result： The expressions of ETS-1 were up regulated by estrogen （ FP = 3.84, P 〈 0. 05 ）, reversely, the expressions of ETS-1 were down regulated with the increasing concentrations of fulvestrant （ FP = 7. 01, P 〈 0. 01 ）. The expressions of ETS-1 （FP = 3.72, P 〈 0.05 ） and VEGF （ FP = 3.68, P 〈 0.05 ） at protein level were increased by estrogen, while, the expressions of ETS-1 （ FP = 4.37, P 〈 0.05 ） and VEGF （ FP = 4.09,P 〈 0.05 ） at protein level were decreased with the addition of fulvestrant. Furthermore, the secretions of VEGF protein in culture media were increased with estrogen（FP =3.91 ,P 〈 0.05 ）, in contrast, VEGF protein concentrations were declined with the supplement of fulvestrant in the culture media（ FP = 7.34, P 〈 0.01 ）. Conclusion：The expressions of ETS-1 and VEGF can be regulated by estrogen and the expression of VEGF is related to ETS-1 in endometrial carcinomal cell line Ishikawa.

关 键 词：雌激素 雌激素受体阻滞剂 子宫内膜肿瘤 ISHIKAWA细胞 ETS-1 血管内皮细胞生长因子 

分 类 号：R737.33[医药卫生—肿瘤]