nm23-H1基因表达对人宫颈癌细胞增殖的影响及与临床病理关系的研究  

Clinical relation of the expression of nm23-H1 gene and the effect of it's transfection on proliferation of human cervical cancer cells

作　　者：杨琰[1] 卢实[1] 李茗[1] 王泽华[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院妇产科,武汉430022

出　　处：《现代妇产科进展》2009年第4期260-264,共5页Progress in Obstetrics and Gynecology

摘　　要：目的:探讨nm23-H1基因稳定表达对人宫颈细胞增殖能力的影响,及其表达与临床特点的关系。方法:用免疫组织化学法检测nm23-H1基因在宫颈癌组织的表达。将真核表达载体pcDNA3.1-nm23-H1转染入宫颈癌细胞系HeLa、SiHa,G418稳定筛选,用Western印迹法、免疫组化法鉴定转染前后细胞中nm23-H1基因的蛋白表达,噻唑蓝(MTT)法检测转染前后细胞体外增殖能力的变化。结果:在宫颈鳞癌组织中,nm23-H1基因阳性表达率随FIGO临床分期的升高有下降趋势,Ⅲ期nm23-H1阳性表达率明显低于Ⅱ期,Ⅱ期阳性表达率明显低于I期(P<0.01);分化程度高的组织阳性表达率显著高于分化程度低的组织(P<0.01);有淋巴结转移者其原发灶阳性表达率明显低于无淋巴结转移者(P<0.01)。宫颈腺癌组织中nm23-H1基因阳性表达率与FIGO临床分期,分化程度,淋巴结转移均无明显的相关性(P>0.05)。在pcDNA3.1-nm23-H1转染组中,Si-Ha、HeLa细胞nm23-H1蛋白表达水平明显增加,未转染组和空载体对照组未见nm23-H1高表达。MTT法所绘生长曲线显示,SiHa-nm23(转染组)细胞与SiHa-3.1(空载体组)、SiHa(空白组)细胞比较,生长速度明显抑制,差异均有统计学意义(P<0.01),而HeLa(空白组)、HeLa-nm23(转染组)、HeLa-3.1(空载体组)三组细胞生长速度均无明显变化(P>0.05)。结论:nm23-H1参与了宫颈鳞癌的发生、发展过程,是判断宫颈鳞癌淋巴结转移和预后的重要标志物,而nm23-H1基因对不同宫颈癌细胞体外增殖能力的影响有明显差异。Objective：To explore the expression of nm23-H1 and correlation with proliferation of human cervical carcinoma cells and their significance with clinical pathological characteristics. Methods： Immunohistochemical method was usedto determine the expression of nm23- H1 in cervical carcinoma tissues. A mammalian expression vector pcDNA3.1-nm23-H1 was transfected into the human cervical carcinoma cell lines SiHa and HeLa. Western-blot and immunohistochemical method was used to analyze the expression levels of nm23-H1 protein. MTT assay were used to detect the ability of proliferation in ceils. Result：In cervical squamous carcinoma tissues, the positive expression rate of nm23-H1 was decreased with the rising of the FIGO clinical stages and increased with the rising of the degree of the differentiation. The positive rate of stageⅢ was significantly lower than that of stage Ⅱ, and stage Ⅱ was significantly lower than that of stage Ⅰ （P 〈0. 01 ）. The expression positive rate with lymph node metastasis was significantly lower than that of nonmetastasis （P 〈 0. 01 ）. But in adenocarcinoma tissues, the positive expression rate of nm23-H1 was not apparent correlation to the FIGO clinical stages ,the degree of the differentiation and the positive rate with lymph node metastasis（P 〉0.05 ）. Exogenous nm23-H1 had successfully been transferred into SiHa and HeLa cells and obtained permanent protein expression. The growth speed of SiHa-nm23 was apparent slower than the other two groups, there was significant difference between them （P 〈 0.01 ） ,but there wasn＇t difference in the growth speed of HeLa-nm23,HeLa-3.1 and HeLa cell lines. Conclusion：nm23-H1 may plan an important role in the development and progression of squamons cell carcinoma of cervical tissues and is an indicator of metastasis potential and prognosis. The effect of nm23-H1 transfection on proliferation of the dif- ferent human cervical carcinoma cell lines is remarkably different.

关 键 词：宫颈肿瘤 基因nm23-H1 基因转染 细胞增殖 

分 类 号：R737.33[医药卫生—肿瘤]