BBP对小鼠睾丸IL-1βmRNA和TNF-αmRNA表达的影响  

The effects of butyl benzyl phthalates on IL-1β and TNF-αmRNA expression in mouse testis

作　　者：吴丹[1] 唐蓓[1] 杨迪[1] 娄小华[1] 杨旭[1] 丁书茂[1] 袁均林[1] 

机构地区：[1]华中师范大学生命科学院环境生物学实验室,武汉430079

出　　处：《环境科学学报》2009年第5期1063-1067,共5页Acta Scientiae Circumstantiae

基　　金：国家科技支撑计划项目(No.2006BAI19B05;2006BAJ02A10)~~

摘　　要：为了探讨邻苯二甲酸丁苄酯(BBP)对小鼠睾丸炎症性细胞因子IL-1β和TNF-α的基因表达的影响,以性成熟的雄性昆明小鼠为实验材料,经不同浓度BBP染毒12d后取其睾丸组织提取总RNA,运用半定量RT-PCR方法分析BBP对小鼠睾丸IL-1β和TNF-α表达水平的影响.实验结果表明,与对照组相比,125mg·kg-1的BBP染毒组小鼠睾丸IL-1β的转录稍有上调,但无显著性差异;250mg·kg-1、500mg·kg-1和1000mg·kg-1的BBP染毒组小鼠睾丸IL-1β的转录呈显著上调(p<0.05或<0.01).TNF-α的转录随所给BBP剂量的增高而上升,呈剂量-效应关系;250mg·kg-1、500mg·kg-1和1000mg·kg-1的BBP染毒组小鼠睾丸TNF-α的转录均显著上调(p<0.05或<0.01).由此可见,较高的BBP暴露水平可使小鼠睾丸内细胞因子IL-1β和TNF-α的转录水平明显上升,从而表达增强,这可能是BBP对小鼠雄性生殖毒性的重要机制之一.The effect of butyl benzyl phthalates （BBP） on gene expression of IL-1βand TNF-α in testes was explored using Kunming purebred mice as experimental animal model. Total RNA was extracted from the testis after exposure to different concentrations of BBP for 12 days. RT-PCR was applied to examine the transcriptional changes of IL-1βand TNF-α. The results showed that 125 mg· kg^-1 BBP up-regulated IL-1β slightly but not significantly compared with the control groups. The groups exposed to 250 mg· kg^-1 , 500 mg· kg^-1 and 1000 mg· kg^-1 showed a significant increase of IL- 18 expression （p 〈 0.05 and p 〈 0.01 ）. The expression of TNF-αinereased with the BBP dose, and there was a dose-effect relationship. Compared with the control groups, 250 mg· kg ^-1 , 500 mg· kg ^-1 and 1000 mg· kg ^-1 BBP up-regulated TNF-α significantly （p 〈 0. 05 and p 〈 0.01 ）. These results suggest that high-concentration BBP could increase the expressions of IL-β.and TNF-α in the testis of mice at the transcription level, which may be the functional mechanism of male reproductive and developmental toxicity induced by BBP.

关 键 词：邻苯二甲酸丁苄酯 雄性生殖毒性 IL-1Β TNF-Α RT-PCR 

分 类 号：X171.5[环境科学与工程—环境科学]