高效液相色谱法检测淀粉类食品中丙烯酰胺的方法研究  

Determination of acrylamide in starch food by solid phase extraction and high performance liquid chromatography

作　　者：茅力[1] 陆晓梅[1] 杨叶[1] 陈景衡[1] 叶洋[1] 傅明慧[1] 潘斐[1] 

机构地区：[1]南京医科大学公共卫生学院,南京210029

出　　处：《中国卫生检验杂志》2009年第4期724-725,774,共3页Chinese Journal of Health Laboratory Technology

基　　金：南京医科大学科技发展基金重点项目资助课题(08NMUZ010)

摘　　要：目的：研究高效液相色谱法检测淀粉类食品中的丙烯酰胺。方法：用纯水提取食品中丙烯酰胺,经化学净化、固相萃取对样品液进行纯化,高效液相色谱法测定。结果：丙烯酰胺在0.02-10.00μg/ml浓度范围内线性良好,相关系数r=0.9993,方法的检测限为0.005μg/ml,样品加标回收率为87.1%-102.2%,样品测定值日内相对标准偏差（RSD）（n=5）为1.45%,日间相对标准偏差（RSD）（n=5）为1.63%。结论：该方法测定食品中丙烯酰胺具有操作简单、干扰少、快速、准确可靠等特点,可以用于大量样品的丙烯酰胺测定。Objective：To study the method for determination of acrylamide in starch food by high performance liquid chromatography. Methods：Acrylamide in food was extracted by water, purified by solid phase extraction, then detected by high performance liquid chromatography. Results ：The calibration curve was linear in the ranges of 0. 02 -10.00 μg/ml, the detection limit was 0. 005 μg/ml. The recoveries were 87.1% - 102.2%. The RSD of inter - day and intra - day precisions was 1.45% and 1.63%, respectively. Conclusion ：The method for determination of acrylamide in starch food is simple, easy to operate, less interfered, accurate and can be used in research of acrylamide in foodstuff.

关 键 词：食品 丙烯酰胺 高效液相色谱法 固相萃取 

分 类 号：O657.72[理学—分析化学]