齐留通对胃癌SGC-7901细胞生长的影响  

Zileuton,a 5-LOX inhibitor,inhibits proliferation and induces apoptos is of SGC-7901 gastric cancer cells

作　　者：潘敏[1] 刘纯伦[1] 方晓杰[1] 石建华[1] 

机构地区：[1]重庆医科大学附属第一医院消化内科,重庆400016

出　　处：《重庆医科大学学报》2009年第5期562-566,共5页Journal of Chongqing Medical University

基　　金：重庆市卫生局课题(05-2-155)。

摘　　要：目的:观察5-脂氧合酶抑制剂齐留通对胃癌SGC-7901细胞增殖、凋亡的影响,初步探讨齐留通影响胃癌细胞生长的机制。方法:MTT法测定齐留通对胃癌SGC-7901细胞增殖的影响;TUNEL染色法计数细胞凋亡率;RT-PCR和免疫细胞化学检测齐留通处理前后SGC-7901细胞Bax、Bcl-2和caspase-3 mRNA和其蛋白表达的改变。结果:齐留通以时间剂量依赖的方式抑制SGC-7901细胞生长;TUNEL染色法显示6.2×10-5mol/L、3.1×10-5mol/L齐留通作用72h后细胞凋亡率为19.6%±6.3%和28.9%±2.3%,均显著高于对照组0.6%±0.1%(P<0.01);RT-PCR和免疫细胞化学显示6.2×10-5mol/L齐留通作用胃癌SGC-7901细胞72h后,可显著促进Bax、caspase-3 mRNA和其蛋白的表达(P<0.05),抑制Bcl-2 mRNA及其蛋白的表达(P<0.05)。结论:齐留通能抑制胃癌SGC-7901细胞增殖,并通过促进Bax和caspase-3的表达,抑制Bcl-2表达,诱导其凋亡,从而抑制胃癌SGC-7901细胞生长。Objective： To evaluate the efficacy of 5-LOX inhibitor zileuton on the proliferation and apoptosis of human gastric carcinoma cell line SGC-7901, and to explore the mechanisms of apoptosis induced by zileuton. Methods： The proliferation of SGC-7901 cells was detected by thiazolyl blue tetrazolium bromide （MTT）assays. TUNEL staining was used to evaluate the apoptosis of SGC-7901 cells. The changes of Bax, Bcl-2 and caspase-3 mRNA expression were studied by reverse transcription polymerase chain reaction. Bax, Bcl-2 and caspase-3 protein expression were analyzed by immunocytochemistry in SGC-7901 cells treated with zileuton. Results： Zileuton can decrease the proliferation significantly in a dose-dependent and time-dependent manner in SGC-7901 cells. TUNEL staining showed that the apoptosis rate was elevated with the increase of zileuton concentration（P〈0.01 ）. 6.2 × 10^-5 mol/L zileuton effectively inhibited the mRNA and protein expression of Bcl-2 （P〈0.05）, but increased Bax and caspase-3 expression （P〈0.05）. Conclusion： 5 LOX inhibitor zileuton can inhibit proliferation and induce apoptosis of SGC-7901 cells by down regulating the expression of Bcl-2 and up regulating Bax and caspase-3.

关 键 词：齐留通 胃癌 SGC-7901细胞 5-脂氧合酶抑制剂 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]