树脂法分离纯化苦荞麦壳中原花青素的研究  

Separation and Purification of Proanthocyanidins from Tartarian Buckwheat Shell by Resin

作　　者：崔莹[1] 朱清华[1] 杨义听[1] 李涛[1,2] 刘睿[1] 

机构地区：[1]华中农业大学食品科技学院,武汉430070 [2]湖北省产品质量监督检验研究院,武汉430061

出　　处：《中国粮油学报》2009年第4期46-49,共4页Journal of the Chinese Cereals and Oils Association

基　　金：华中农业大学SRF项目;华中农业大学创新基金资助(2006-11)

摘　　要：为纯化苦荞麦壳中原花色素,比较了5种树脂对苦荞麦壳中原花青素的静态吸附、解吸性能,并研究了ADS-17树脂对原花青素动态吸附、解析的最佳条件。结果表明:ADS-17树脂是较理想的吸附树脂,在溶液中ADS-17树脂对原花青素的吸附5 h达到平衡。最佳动态吸附、解析条件是以体积分数70%甲醇为洗脱剂,吸附流速为3 mL/min,上样溶液pH 6.3,层析柱径高比为1∶6,最终产物纯度达到52%。To purify proanthocyanidins from tartarian buckwheat shell by resin, the static adsorption and desorption performance of 5 kinds of resin for proanthocyanidins from tartarian buckwheat shell were compared, and the optimum conditions of the dynamic adsorption and desorption for separating pmanthocyanidins from tartarian buckwheat shell by ADS- 17 resin were studied. Experiment results indicate ADS - 17 resin is the desired adsorbent resin, with which the adsorption equilibrium time is 5 h in this experiment. The optimum conditions of dynamic adsorption and desorption are as follows： 70 % methanol is used as eluant, adsorption velocity is 3 mL/min, pH of sample is controlled at 6.3 and the ratio of chromatography column diameter to height is 1： 6. The purity of the product is 52%.

关 键 词：苦荞麦壳 原花青素 树脂 分离 纯化 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]