不同脂肪酸构成比膳食对大鼠脂联素及其受体表达的影响  

GENE EXPRESSION OF ADIPONECTIN AND ADIPONECTIN RECEPTOR IN RATS FED DIETS WITH DIFFERENT FATTY ACIDS RATIOS

作　　者：彭安芳[1] 毛丽梅[1] 陈艳[1] 丁强[1] 夏亮[1] 章锡平[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院营养与食品卫生系,武汉430030

出　　处：《营养学报》2009年第2期129-131,135,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30571561)

摘　　要：目的探讨不同脂肪酸构成比膳食对大鼠脂联素及其受体表达的影响。方法将72只Wistar大鼠随机分为对照组和5个实验组(A～E),实验组分别设膳食中饱和脂肪酸(SFA)/单不饱和脂肪酸(MUFA)/多不饱和脂肪酸比值(PUFA)(S/M/P)为1:1.7:1.2、1:1:1、2:1:1、1:2:1和1:1:2,喂养12w,测定脂肪组织脂联素(adiponectin,ADPN)、骨骼肌组织脂联素受体1(AdipoR1)、肝脏组织脂联素受体2(AdipoR2)mRNA的表达水平。结果与基础组相比,1:1.7:1.2组和1:1:1组的两种脂肪组织和2:1:1组的皮下脂肪组织脂联素mRNA表达显著降低(P<0.05),其中1:1.7:1.2组下降程度较高(P<0.001)。与1:1.7:1.2组比较,其余各实验组脂联素表达水平均显著升高(P<0.05),以1:1:2组的升高程度最高(P<0.001)。各实验组AdipoR的表达水平与基础对照组并无明显差别(P>0.05)。结论高脂饮食降低脂肪组织脂联素mRNA表达水平,不饱和脂肪酸对促进脂联素基因表达和分泌有明显作用,其中PUFA的作用较MUFA显著。Objective To study the expression of adiponectin and adiponectin receptors mRNA in rats fed diets with different fatty acid ratios. Method Seventy two male Wistar rats were randomly divided into control group and 5 experimental groups （A-E）. The experimental group were fed diets with saturated fatty acids （SFA）/monounsaturated fatty acid （MUFA）/polyunsaturated fatty acid ratio （PUFA） （S/M/P） 1：1, 7：1.2, 1：1：1, 2：1：1, 1：2：1 and 1：1：2. The adipose tissue adiponectin （ADPN）, skeletal muscle adiponectin receptor 1 （AdipoR1） and liver adiponectin receptor 2 （AdipoR2） mRNA expression levels were determined after 12 w. Result The mRNA levels of adiponectin in both adipose tissues of group 1：1.7：1.2 and 1：1：1 were significantly lower as compared with the control group （P〈0.05） , as well as the mRNA level of adiponectin in subcutaneous adipose tissues of group 2：1：1. The mRNA levels of adiponectin in other experimental groups were significantly higher as compared with the control （P〈 0.05 ）, the highest in group 1：1：2 （P〈0.001）. There was no significant difference in AdipoR mRNA levels between the control group and other groups. Conclusion The adiponectin mRNA level in adipose tissue was decreased in rats fed high-fat diet. Unsaturated fatty acid increased adiponectin mRNA level, more conspicuous in PUFA than MUFA.

关 键 词：脂肪酸构成比 脂联素 脂联素受体 

分 类 号：R151[医药卫生—营养与食品卫生学]