微电极阵列细胞阻抗传感器研究  

Micro-Electrode Array Based Biosensor for Cellular Impedance Detection

作　　者：刘清君[1,2] 胡朝颖[1] 叶伟伟[1] 张威[1] 徐莹[1] 王平[1,2] 

机构地区：[1]浙江大学生物医学工程与仪器科学学院,生物医学工程教育部重点实验室,生物传感器国家专业实验室,杭州310027 [2]中国科学院,传感技术联合国家重点实验室,上海200050

出　　处：《传感技术学报》2009年第4期447-450,共4页Chinese Journal of Sensors and Actuators

基　　金：国家自然科学基金资助(30700167);浙江省自然科学基金资助(Y2080673);传感技术联合国家重点实验室基金资助(Skt0702)

摘　　要：贴壁生长在微电极表面的细胞可引起贴壁电极界面阻抗的改变,从而可以获得细胞生理功能相关的生物信息。本研究采用微机械加工技术,在硅基底上设计了直径为20～50μm的20通道金微电极阵列(micro-electrode array,MEA),用以构建能实时、连续、定量跟踪哺乳动物细胞形态和增殖分化改变的细胞阻抗测试传感器(electric cell-substrate impedance sensing,ECIS),用于细胞与电极间的阻抗测试研究。通过对培养在微电极表面24h的成骨细胞Saos-2细胞系的阻抗谱测量发现,其阻抗值增加集中在中频102～104Hz之间,本结果符合细胞阻抗传感测量的理论模型分析。因此,该微电极阵列细胞阻抗传感器的研究,为进一步的细胞生理和药物分析等研究奠定了良好的基础。When adherently growing cells cultured on the surface of the micro-electrodes, impedance detection can provide information related to cellular physiological functions. To establish electric cell-substrate impedance sensing （ECIS） system for detecting of culture morphology and proliferation of cultured mammal cells in a real time, non-invasive and quantitative way, a cell based biosensor with 20 channel gold micro-electrode arrays （MEA） was designed in this study. Based on micromechanical systems, diameters of these electrodes were fabricated about 20-50 micron on silicon wafe. After human osteoblasts Saos-2 cell lines were cultured on the surface of the electrodes about 24 h later, the impedance increase was mainly focused on 10^2- 10^4 Hz of the frequency spectroscopy. The impedance detecting result was well according with analysis of the impedance models of the theoretic analysis. Therefore, this cell-based impedance biosensor with micro-electrodes can be used for studies of cell physiologies and drug screenings.

关 键 词：细胞传感器 微电极阵列 阻抗 细胞阻抗测试传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]