西藏小型猪ESR基因PvuⅡ多态性与生长繁殖性能相关分析  

Correlation analysis of the relationship between PvuⅡ polymorphisms of ESR gene and growth and reproduction traits of Tibet mini-pig

作　　者：曾昭智[1] 江涛[1] 张锦红[1] 顾为望[2] 

机构地区：[1]广东药学院实验动物中心,广东广州510006 [2]南方医科大学实验动物中心,广东广州510515

出　　处：《广东药学院学报》2009年第2期192-195,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省科技计划项目(2005B60302007)

摘　　要：目的了解ESR基因PvuⅡ位点在西藏小型猪中的多态性分布情况;研究其在西藏小型猪上的遗传效应,为西藏小型猪生长繁殖性状的分子遗传标记辅助选择提供实验依据。方法记录44头西藏小型猪母猪繁殖性能,并对其所产仔猪的生长性能进行测定,采用PCR-RFLP方法对ESR基因PvuⅡ位点进行多态性与生长繁殖性能进行相关性分析。结果西藏小型猪ESR候选基因PvuⅡ位点表现出多态性,为AA、AB、BB型,A、B等位基因频率特征。初产胎的产仔数在不同基因型间差异存在显著性,不同基因型间产仔数呈BB>AB>AA的趋势,初产胎和经产胎的母猪其他指标在不同基因型间差异均没有显著性。结论提高西藏小型猪猪群ESR基因PvuⅡ位点B等位基因频率,有利于提高西藏小型母猪的产仔数。Objective To obtain the information on Pvu Ⅱ polymorphism distribution of ESR gene and study its genetic effect on Tibet mini-pigs to provide experimental evidence for molecule genetic marker selection of growth and reproductive traits. Methods Growth and reproduction traits of 44 Tibet mini-pigs were recorded. The Pvu Ⅱ polymorphism of ESR gene was detected by PCR-RFLP and the relationship between polymorphism and growth and reproduction performance was analyzed. Results The genotypes were AA, AB, BB with A and B were alleles. TBN （total birth number）of the primiparous sows was significantly different between different genotypes and the tendency of TBN between different genotypes was BB 〉 AB 〉 AA, other index between primiparous sows and parous sows showed no significant difference. Conclusion The study results demonstrated that improving allele frequency of B allele of ESR gene might be helpful to improve TBN of Tibet mini-pigs.

关 键 词：西藏小型猪 ESR基因 多态性 生长繁殖性能 相关分析 

分 类 号：R-332[医药卫生]