乳源活性肽抗疲劳作用的实验研究  

Study on Anti-fatigue Effect of Milk Protein-derived Peptide

作　　者：江小云[1] 潘道东[1] 

机构地区：[1]南京师范大学乳品生物技术研究所,江苏南京210097

出　　处：《食品科学》2009年第9期191-194,共4页Food Science

基　　金："十一五"国家科技支撑计划项目(2006BAD27B09);国家高技术研究发展计划项目(2007AA10Z320);江苏省高校高新技术产业发展项目(JHB06-11)

摘　　要：本实验研究酶解乳蛋白后产生的小分子肽对小鼠抗疲劳能力的影响。实验用酶水解乳蛋白产肽,利用超滤及SephadexG-15凝胶柱层析分离出6个组分,以小鼠负重游泳时间为指标从这6个组分中筛选出1个抗疲劳作用最优组分,并通过测定小鼠负重游泳时间、小鼠运动后血清尿素氮、肝糖原含量和游泳前、游泳后0min和20min血清乳酸含量的变化,对其抗疲劳功能进行了评价。结果显示乳蛋白水解肽能显著增加小鼠负重游泳时间,降低小鼠运动后血清尿素氮含量,提高了小鼠肝糖原含量并能降低游泳后血清乳酸的增加量。表明乳蛋白水解肽目标组分能够提高小鼠的抗疲劳能力。In the present study, lactose-removed milk protein was hydrolyzed into peptides with chymotrypsin and trypsin together, and then after removing the substances with over 6000 D molecular weight by ultra-filtration, the residual hydrolysates were separated into 6 fractions （a, b, c, d, e, and f） by Sephadex G- 15 gel chromatography. Kunming male mice were administered the above 6 fractions respectively by garage for 28 days, and with the loaded swimming time as evaluation index, the best antifatigue peptide was chosen from the 6 fractions. Furthermore, through the determination of loaded swimming time, content of serum urea nitrogen after swimming, liver glycogen level, and serum lactate concentration before swimming of mice, its antifatigue ability was evaluated. The results showed that the fraction c is the best anti-fatigue peptide. It can significantly promote the loaded swimming time and liver glycogen level of mice, and reduce the contents of serum urea nitrogen and lactate of mice after swimming. It was concluded that the fraction c can improve the anti-fatigue ability of mice.

关 键 词：乳源活性肽 抗疲劳作用 

分 类 号：TQ936.16[化学工程]