白术多糖对大鼠脑缺血再灌注期炎症反应的影响  

Effect of Atractylodes macrocephala Koidz Polysaccharide on Brain Inflammation during Focal Cerebral Ischemia Reperfusion in Rats

作　　者：王光伟[1] 丰昀[2] 刘永乐[3] 李加兴[4] 邱细敏[1] 

机构地区：[1]湖南师范大学医学院,湖南长沙410006 [2]长沙市中心医院,湖南长沙410004 [3]长沙理工大学生物与食品工程学院,湖南长沙410015 [4]吉首大学食品科学研究所,湖南吉首416000

出　　处：《食品科学》2009年第9期216-218,共3页Food Science

基　　金：湖南省自然科学基金项目(08JJ3024);长沙市科学技术局资助项目(K069062-12)

摘　　要：目的:探讨局灶性脑缺血再灌注早期应用白术多糖治疗对脑组织炎症反应的影响。方法:采用大脑中动脉线栓/再灌注模型,84只雄性SpragueDawley大鼠随机分为对照组、再灌注组、蒸馏水组及白术多糖组,每组再根据不同再灌注时间随机分为3个亚组,取各组动物脑缺血区组织,分别检测其髓过氧化物酶(MPO)活性,做细胞间黏附分子-1(ICAM-1)免疫组化染色,光镜下计数ICAM-1阳性血管数。结果:白术多糖组各时间点ICAM-1阳性血管数均明显少于再灌注组及蒸馏水组相应时间点(p<0.01)。白术多糖组各时间点MPO活性均明显低于再灌注组及蒸馏水组相应时间点(p<0.01)。结论:再灌注早期小剂量应用白术多糖能明显减少缺血区中性粒细胞浸润及ICAM-1的表达,有助于改善炎症反应引起的再灌注损伤。Objective： To study the effect of polysaccharide extracted from Atractylodes macrocephala Koidz on brain inflammatory response during focal cerebral ischemia reperfusion in rats. Methods： Focal cerebral ischemia was induced in anesthetized rats by using a middle cerebral artery occlusion （MCAO） model. Eighty-four male Sprague Dawley rats were randomly divided into four groups： sham group, reperfusion group, distilled water group and Atractylodes macrocephala Koidz polysaccharide group. Then three subgroups of each rat group were obtained at 1 h of MCAO with different time intervals （6 , 12 and 24 h） of reperfusion. Myeloperoxidase （MPO） activity assay and calculation of blood vessels with positive immunohistochemical staining of intercellular adhesion molecule-1 （ICAM-1） were performed in the ischemic brain tissues. Results： The ICAM- 1-positive blood vessels in Atractylodes macrocephala Koidz polysaccharide group are significantly less than those in reperfusion group and distilled water group at different time intervals of reperfusion （p 〈 0.01）. The MPO activity in Atractylodes macrocephala Koidz polysaccharide group is significantly lower than that in reperfusion group and distilled water group （p 〈 0.01）. Conclusion： Atractylodes macrocephala Koidz polysaccharide could dramatically decrease neutrophil infiltration and ICAM-1 expression in the ischemic areas. It might be helpful to ameliorate the reperfusion injury induced by brain inflammatory response.

关 键 词：白术多糖 脑缺血 再灌注 细胞间黏附分子-1 

分 类 号：Q946.3[生物学—植物学]