人肝癌裸鼠皮下-肝原位移植瘤模型的建立  

Study of Using α-cyanoacrylate to Establish the Orthotopic Transplantation Tumor Model From the Subcutaneous Model of Human Hepatocellular Carcinoma in Nude Mice

作　　者：白俊文[1] 苏秀兰[2] 杨成旺 王玉芳[1] 

机构地区：[1]内蒙古医学院附属医院普外科,内蒙古呼和浩特010050 [2]内蒙古医学院临床医学研究中心,内蒙古呼和浩特010050 [3]内蒙古自治区卫生厅,内蒙古呼和浩特010055

出　　处：《内蒙古医学杂志》2009年第3期257-259,F0003,共4页Inner Mongolia Medical Journal

基　　金：国家自然科学基金资助项目(30460146)

摘　　要：目的:使用α-氰基丙烯酸烷基酯建立人肝癌-裸鼠皮下肝原位移植瘤模型。方法:培养对数生长期的BEL-7402人肝癌细胞,接种于裸鼠皮下,形成皮下移植瘤后再移植于裸鼠肝内,利用α-氰基丙烯酸烷基酯建立肝原位移植瘤模型(间接肝原位移植瘤模型)。结果:成功的利用α-氰基丙烯酸烷基酯建立了间接肝原位移植瘤模型。移植瘤成功率达93.3%。结论:与传统的肝原位移植瘤模型制作方法比较,利用α-氰基丙烯酸烷基酯建立间接肝原位移植瘤模型方法简便,成功率高,便于推广使用。Objective： Using α- cyanoacrylate to establish the orthotopic transplantation tumor model from the subcutaneous model of human hepatocellular carcinomas（HCC）in nude mice. Methods： The subcutaneous animal model was established by transplanting BEL - 7402 cell into the subcutaneous of nude mice. Then the orthotopic transplantation tumor model was established by injecting the subcutaneous transplanted tumor into liver（indirect orthotopic model）. Using α - cyanoacrylate to stanch a cut and prevent tumor from falling. It was compared with the traditional indirect orthotopic transplantation model and observed postoperative complications. Results： Using α - cyanoacrylate to establish the indirect orthotopic model propagated stably and easy. The survival rates of the transplantation tumor were 93.3 % . Conclusion： The indirct orthotopic transplantation tumor model by using α- cyanoacrylate to establish is an ideal instrument for study of the metastatic mechanism and screen of anti- metastatic drugs. The method is easy and convenient.

关 键 词：肝细胞瘤移植 裸鼠 原位 α-氰基丙烯酸烷基酯 

分 类 号：R735.7[医药卫生—肿瘤]