芘降解质粒提取方法的研究  

Isolation and Research of Pyrene Degrading Plasmids

作　　者：蔡丽希[1] 许桂芬[1] 

机构地区：[1]莆田学院医学院,莆田351100

出　　处：《生物技术通报》2009年第5期92-95,共4页Biotechnology Bulletin

摘　　要：采用碱裂解法、化学改良方法、CTAB法、酚氯法等4种方法提取从泉州红树林沉积物中筛选出多环芳烃降解菌群YL的质粒,并分别转化至大肠杆菌感受态细胞,然后采用富集筛选的手段,最终确立最佳方案得到一组质粒转化菌群。该转化菌群可以利用芘作为其生长的惟一碳源和能源,并能在21 d内将50 mg.L-1的芘降解85.69%。而受体菌E.coliDH5α的芘降解率仅为2.006%。由此可以证明,降解质粒已成功转化进宿主细胞,并发挥降解作用。YL的质粒含有降解芘的基因。Alkaline lysis, phenol and chloroform extraction ,CTAB and modified chemical methods from chemistry were used for isolation of plasmid DNA from a kind of pyrene degrading bacteria isolated from the sediment of mangrove in Quanzhou city. After plasmid preparation , the plasmid were transformed into E. coli DH5α and found that the transformed bacteria have the ability of pyrene degradation and utilize pyrene as sole carbon sources. The degrading rate of pyrene was 85.69% within 21 days ,compared to the E. coli DH5α that degraded 2. 006% of pyrene only within 21 days. Therefore, the transformed bacteria presented a good pyrene degradation capability, and the plasmids contain the gene that can be able to encode for the degradation of pyrene.

关 键 词：多环烷烃 红树林 芘 降解质粒 

分 类 号：X132[环境科学与工程—环境科学]